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| 溶血磷脂酸上调ROS水平诱导HeLa-S3细胞迁移及相关机制的探讨 | |
| 引用本文： | 姚慕崑,刘玮,任萍萍,吴希美.溶血磷脂酸上调ROS水平诱导HeLa-S3细胞迁移及相关机制的探讨[J].中国药理学通报,2012,28(5):637-640. |
| 作者姓名： | 姚慕崑 刘玮 任萍萍 吴希美 |
| 作者单位： | 1. 浙江省立同德医院,浙江,杭州,310012 2. 浙江大学医学院临床一系,浙江,杭州,310058 3. 浙江大学医学院药理学系,杭州,310058 |
| 基金项目： | 吴阶平医学基金资助课题,国家自然科学基金面上项目,科技部"973"课题,科技部"973"子课题,浙江省杰出青年基金 |
| 摘 要： | 目的探讨溶血磷脂酸(LPA)诱导HeLa-S3细胞迁移能力的机制。方法采用细胞迁移实验测定HeLa-S3细胞的迁移;Western blot检测Akt、P-Akt(Ser473)、PAK1、P-PAK1(Thr423)的表达;采用CM-H2DCFDA检测细胞ROS的生成情况。结果①20μmol·L-1LPA可使细胞内ROS的产生明显增加(P<0.05),采用NAC预处理后能明显的抑制ROS的生成;②LPA可升高P-Akt(Ser473),P-PAK1(Thr423)/PAK1水平,但采用LY294002可以抑制PAK1(Thr423)磷酸化水平的升高和细胞的迁移(P<0.01)。结论溶血磷脂酸通过上调ROS水平来诱导HeLa-S3细胞的迁移,其可能的机制是通过PI3K信号通路活化Akt/PAK1来实现的。 |
| 关 键 词： | 溶血磷脂酸 HeLa-S3细胞 PI3K Akt/PAK1 ROS 细胞迁移 |
Lysophosphatidic acid induced HeLa-S3 cell migration |
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| YAO Mu-kun , LIU Wei , REN Ping-ping , WU Xi-mei.Lysophosphatidic acid induced HeLa-S3 cell migration[J].Chinese Pharmacological Bulletin,2012,28(5):637-640. | |
| Authors: | YAO Mu-kun LIU Wei REN Ping-ping WU Xi-mei |
| Affiliation: | 1.Tongde Hospital,Zhejiang Province,Hangzhou 310012,China;2.Dept of Clinical Medicine,Medical College of Zhejiang University,Hangzhou 310058,China;3.Dept of Pharmacology,Medical College of Zhejiang University,Hangzhou 310058) |
| Abstract: | Aim To explore the mechanisms of lysophosphatidic acid(LPA) in the migration of HeLa-S3 cell.Methods The cell migration experiments were employed to test the migration of HeLa-S3 cells;Western blot was used to analyse Akt,P-Akt(Ser473),PAK1,P-PAK1(Thr423) expression;CM-H2DCFDA was used to test the Intracellular ROS level.Results(1) 20 μmol·L-1 LPA increased the production of intracellular ROS(P<0.05),while the pretreatment with NAC significantly inhibited LPA-induced intracellular ROS generation;(2) LPA elevated-Akt(Ser473)/Akt and P-PAK1(Thr423)/PAK1,while the pretreatment with LY294002 inhibited the increased P-PAK1(Thr423)/PAK1and cell migration.Conclusions LPA induces the migration of HeLa-S3 cells through the up-modulation of ROS level,and its possible mechanisms may be related to activating Akt/PAK1 by PI3K signal pathway. |
| Keywords: | lysophosphatidic acid HeLa-S3 cell PI3K Akt/PAK1 ROS cell migration |
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