过敏性紫癜患儿血清白介素6、白介素8及肿瘤坏死因子α水平和免疫球蛋白的变化

目的探讨血清白介素6、白介素8、肿瘤坏死因子。及免疫球蛋白在过敏性紫癜患儿中的表达及其临床意义。方法采用ELISA法和免疫散射比浊法检测了45例过敏性紫癜患儿(其中20例合并肾脏损害)及43例正常健康儿童的血清白介素6、白介素8、肿瘤坏死因子α及免疫球蛋白水平,分别对过敏性紫癜有无合并肾损害及过敏性紫癜患儿与健康儿童细胞因子和免疫球蛋白水平进行比较;观察是否存在相关关系。结果HSP患儿的血清IL-6、IL-8及TNF-α水平高于正常对照组(P<0·01);无肾损害HSP组与HSPN组的血清IL-6、IL-8水平均无统计学差异,HSPN组TNF-α水平高于无肾损害HSP组;HSP患儿IgA、IgE含量明显高于正常对照组(P<0·01),IgG含量下降(P<0·01),IgM无明显差别(P>0·05);无肾损害HSP组与HSPN组的IgA、IgM、IgG水平无统计学差异(P>0·05),HSPN组的IgE水平高于无肾损害HSP组(P<0·05);HSP患儿的血清TNF-α水平与IL-8存在中等程度正相关关系(r=0·524,P<0·01)。HSP患儿的血清TNF-α水平与IL-6存在中等程度正相关关系(r=0·670,P<0·01)。结论HSP/HSPN的发病过程存在细胞因子和异常水平免疫球蛋白的参与,HSPN的发生与TNF-α异常有相关性。

Study of serums IL-6,IL-8,TNF-α and immunoglobulin in children with Henoch-Schonle in purpura

Objective To investigate the changes of serum levels of IL-6、IL-8、TNF-α and immunoglobulin, and probe their clinical significance in children with HSP /HSPN. Methods To examine the serum levels of IL-6、IL-8、TNF-α and immunoglobulin of 45 children with HSP/HSPN by ELISA and using full-automatic biochemistry analyzer. Analyze the changes and the correlation between the aforementioned cytokines and immunoglobulin in children with HSP /HSPN. Results Serum levels of IL-6、IL-8 and TNF-α of the patients with HSP were nigher than those of the normal control group (P<0. 01). No significant difference was found between non-renal involvement HSP and HSPN groups on serum levels of IL-6 and IL-8, but the serum level of TNF-α of HSPN was higher than that of non-renal involvement HSP. Serum levels of IgA and IgE of the patients with HSP were higher than those of the normal control group (P<0.01), Serum levels of IgG of the patients with HSP were lower than those of the normal control group (P<0.01), IgM were not significant differencebetween the two groups (P> 0.05). No significant difference was found between non-renal involvement HSP and HSPN groups on serum levels of IgA、 IgM、 IgG, but the serum level of IgE of HSPN was higher than that of non-renal involvement HSP (P<0.05). The serum level of TNF-α was positively correlated to the serum levels of IL-6 and IL-8 of patients with HSP (r=0. 670, P<0. 01; r=0. 524, P<0. 01). Conclusion The results imply the cytokines and immunoglobulin may play role in the pathogeny of HSP, the TNF-α cause resulting in the HSPN, The mechanism that results in the HSP kidney injure needs a further more study.