蓬莪术的离体快繁研究

目的]探索蓬莪术的离体快繁技术。方法]以蓬莪术嫩叶、块茎和根为外植体,接种在以MS为基本培养基,外加不同浓度激素组合的培养基上进行愈伤组织诱导、增殖和分化培养;以幼芽为外植体,进行幼芽的增殖、生根培养和移栽等。结果]诱导蓬莪术叶、块茎和根的愈伤组织形成的最适培养基为MS＋2,4-D1.0 mg/L＋6-BA0.5～1.0 mg/L＋NAA0.3 mg/L,其中蓬莪术叶诱导率最高,达96%;愈伤组织增殖和分化培养还有待进一步研究。幼芽最适增殖培养基为MS＋6-BA3.0 mg/L＋NAA0.1 mg/L＋KT1.0 mg/L,其增殖倍数达3.5以上;而生根培养基以1/2 MS＋NAA1.0 mg/L＋6-BA0.5 mg/L为最佳,生根率达100%,其移栽成活率达85%以上。结论]该研究结果为蓬莪术的快速繁殖及工厂化生产奠定了基础。

Study on Rapid Propagation of Curcuma phaeocaulis Valetion in vitro

Objective] The study aimed to explore the rapid propagation technology of Curcuma phaeocaulis Valetion in vitro.Method] The tender leaves,stem tubers and roots of C.phaeocaulis were used as explants to study the effects of different concentration and kinds of hormone combinations on callus induction,proliferation,differentiation,bud proliferation,rooting culture and transplanting.Result] The results showed that optimal callus induction medium for inducing the callus formation of C.phaeocaulis leaves,stem tubers and roots was MS+1.0 mg/L 2,4-D+0.5-1.0 mg/L 6-BA +0.3 mg/L NAA.The highest induction rate of C.phaeocaulis leaves was 96%.The callus multiplication and differentiation culture need furture research.The optimal medium for tender bud multiplication was MS+ 6-BA 3.0 mg/L + NAA 0.1 mg/L + KT 1.0 mg/L,whose multiplication multiple reached over 3.5.The optimal rooting medium was 1/2 MS + NAA 1.0 mg/L +6-BA 0.5 mg/L,with the rooting rate of 100%.And the transplanting survival rate reached over 85%.Conclusion] The research results laid the foundation for the rapid propagation and industrialized production of C.phaeocaulis.