Association between biomass formation and the prognosis of infective endocarditis due to Staphylococcus aureus |
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| Affiliation: | 1. Servei de Malalties Infeccioses, Hospital Universitari Vall d’Hebron, Universitat Autònoma de Barcelona, Barcelona, Spain;2. Red Española de Investigación en Patología Infecciosa, Instituto de Salud Carlos III, Madrid, Spain;3. Servicio de Microbiología, Hospital Universitario 12 de Octubre, Madrid, Spain;4. Unitat d’Epidemiologia Cardiovascular, Servei de Cardiologia, Hospital Universitari Vall d’Hebron, CIBERESP, Barcelona, Spain;5. Servei de Microbiologia, Hospital Universitari Vall d’Hebron, Universitat Autònoma de Barcelona, Barcelona, Spain;1. Servicio de Laboratorio Central, Hospital Universitario de Canarias, Tenerife, España;2. Servicio de Aparato Digestivo, Hospital Universitario de Canarias, Tenerife, España;3. Servicio de Farmacia, Hospital Universitario de Canarias, Tenerife, España;1. Servicio de Medicina Interna, Complejo Hospitalario de Navarra, Pamplona, Spain;2. Infectious Diseases Service, Hospital Clínic de Barcelona, Universidad de Barcelona, Spain;1. Área de Enfermedades Infecciosas y Microbiología Clínica, Laboratorio de Biofilms Microbianos, Clínica Universidad de Navarra, España;2. Instituto de Investigación Sanitaria de Navarra (IdiSNA), Pamplona, España;3. Unidad de Microbiología Experimental, Centro de Investigación La Fe, Valencia, España;1. Instituto de Medicina Tropical “Alexander von Humboldt”, Universidad Peruana Cayetano Heredia, Lima, Peru;2. Facultad de Ciencias y Filosofía, Universidad Peruana Cayetano Heredia, Lima, Peru;3. ISGlobal, Hospital Clínic - Universitat de Barcelona, Barcelona, Spain;4. University of Texas Health Science Center at Houston, School of Public Health, Houston, USA |
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| Abstract: | IntroductionThe aim of this study was to evaluate the association between biomass formation and the clinical characteristics and prognosis of Staphylococcus aureus infective endocarditis (IE).MethodsWe prospectively studied 209 S. aureus strains causing IE. Biomass formation was examined using the crystal violet assay and quantified spectrophotometrically. The average (SD) optical density of the biomass was compared for each clinical, microbiological (methicillin-resistance, vancomycin MIC ≥ 1.5 μg/ml) and molecular (clonal complex, agr type and agr dysfunction) variable according to their presence or absence. The primary clinical endpoints studied were in-hospital death, severe sepsis, persistent bacteraemia, symptomatic peripheral embolisms and prosthetic valve IE.ResultsMean age was 66.1 years, 61.5% of patients were male and the median age-adjusted Charlson comorbidity index was 5 points (IQR 3–8). In-hospital mortality was 37.3%. Strains belonging to CC5 and CC22 had optical biomass densities [mean (SD) 1.573 (1.14) vs 0.942 (0.98) p < 0.001 and 1.720 (0.94) vs 1.028 (1.04) p = 0.001, respectively]. Strains belonging to CC5 and CC22 had significantly higher optical biomass densities [1.369 (1.18) vs 0.920 (0.93) p = 0.008]. No statistically significant differences were found in the clinical endpoints studied.ConclusionsHigh biomass production was associated with CC5 and CC22 but not with higher hospital mortality, septic complications, type of endocarditis, methicillin-resistance, elevated vancomycin MIC or agr dysfunction. |
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| Keywords: | Infective endocarditis Biofilm Biomass Optical density Prognosis Endocarditis infecciosa Biopelícula Biomasa Densidad óptica Pronóstico |
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