| 乌梅提取液对李斯特菌抑菌机理 |
| |
| 引用本文： | 耿 飞,王 伟,周 涛.乌梅提取液对李斯特菌抑菌机理[J].食品科学,2011,32(15):88-93. |
| |
| 作者姓名： | 耿 飞 王 伟 周 涛 |
| |
| 作者单位： | 南京师范大学金陵女子学院食品科学与工程系 |
| |
| 基金项目： | 江苏省农业科技自主创新基金项目(CX(09)627 |
| |
| 摘 要： | 运用流式细胞仪研究乌梅提取液对李斯特菌的抑菌机理。首先通过抑菌实验确定乌梅提取液对李斯特菌的最小抑菌浓度(MIC)为0.625mg/mL，然后用1、2、4倍MIC的乌梅提取液对李斯特菌进行处理，通过流式细胞仪对其前向散射光(FSC)变化、DNA含量变化、细胞膜电位变化、细胞周期变化和Annexin V-FITC/PI双标记法、细胞内Ca2+含量变化指标进行检测，探索抑菌机理。结果表明，乌梅提取液通过破坏李斯特菌的细胞膜系统和阻滞DNA的合成抑制其生长繁殖。
|
| 关 键 词： | 流式细胞仪 乌梅 李斯特菌 抑菌机理 |
Antibacterial Mechanisms of Fructus mume Extract against Listeria innocua |
| |
| GENG Fei,WANG Wei,ZHOU Tao.Antibacterial Mechanisms of Fructus mume Extract against Listeria innocua[J].Food Science,2011,32(15):88-93. |
| |
| Authors: | GENG Fei WANG Wei ZHOU Tao |
| |
| Affiliation: | (Department of Food Science and Engineering, Ginling College, Nanjing Normal University, Nanjing 210097, China) |
| |
| Abstract: | The antibacterial mechanisms of ethanol extract from Fructus mume (prepared by reflux extraction with 95% ethanol and subsequent rotary evaporation until a concentration of 1 g/mL, namely raw material weight/condensed extract volume ratio = 1:1) against Listeria innocua were assayed by flow cytometry. The minimum inhibitory concentration (MIC) of the extract against L. innocua was 0.625 mg/mL. In the presence of the extract at different concentrations such as 1, 2 and 4 MICs, the changes of forward scatter (FSC) light, DNA content, membrane potential, cell cycle, Ca2+ and Annexin V-FITC/PI double staining of L. innocua were analyzed by flow cytometry. The results showed that the extract could inhibit L. innocua by destroying cell membrane system and blocking DNA synthesis. |
| |
| Keywords: | flow cytometry Fructus mume L innocua antibacterial mechanism |
|
| 点击此处可从《食品科学》浏览原始摘要信息 |
|
点击此处可从《食品科学》下载全文 |
|
