端粒酶在宫颈癌及其癌前病变组织中的表达及意义

目的探讨端粒酶在宫颈癌及癌前病变组织中的表达及其在宫颈癌发生、发展过程中的作用。方法采用免疫组织化学EnVision二步法,检测30例浸润性宫颈癌（ICC）、90例宫颈上皮内瘤变（CIN）和10例正常宫颈组织标本中端粒酶的表达。结果端粒酶在正常宫颈组织、CINⅠ、CINⅡ、CINⅢ及浸润性宫颈癌中的阳性表达率分别为30.0%、40.0%、43.3%、70.0%和93.3%。端粒酶表达率浸润性宫颈癌高于CINⅢ,CINⅢ高于CINⅡ,差异均有统计学意义（P〈0.05）;CINⅡ与CINⅠ,CINⅠ与正常宫颈组织比较,差异均无统计学意义（P〉0.05）。结论端粒酶的表达与宫颈癌的发生、发展密切相关。端粒酶可作为宫颈癌癌前病变转归的预测指标。

Expression of Telomerase in Cervical Carcinoma and Cervical Intraepithelial Neoplasia and its Significance

Objective To investigate the expression of telomerase in cervical carcinoma and cervical intraepithelial neoplasia and the role during the development of cervical carcinoma. Methods The expression of telomerase in 30 cases of invasive cervical carcinoma（ICC） ,90 cases of cervical intraepithelial neoplasia（CIN） , and 10 cases of normal cervices were detected by immunohistochemical technique. 90 cases of CIN were divided into 3 groups： CIN I ,CIN II and CIN III ,each of them containing 30 cases. Results The positive rates of telomerase in normal cervices, CIN I, CIN II , CIN III and invasive cervical carcinoma were 30.0% ,40.0% ,43.3% ,70.0% ,93.3% respectively. The positive rate of telomerase in ICC was significantly higher than that of CIN III （ P 〈 0.05 ）. And those in CIN III was significantly higher than that of CIN II （ P 〈 0.05 ）, but there were of no significant difference in CIN II and CIN I ,CIN I and normal cervical tissues （ P 〉 0.05 ）. Conclusion There is a close relationship between high expression of telomerase and development of cervical carcinoma. Telomerase may be a valuable indicater of the prognosis for premalignant cervical lesion.