量子点免疫标记技术在肺癌组织芯片上的应用

目的:探讨新的标记试剂——量子点(QDs)在肺癌组织芯片上进行不同蛋白的检测并评价其效果。方法:在肺癌组织芯片上,利用QDs标记的链霉亲和素复合物(QDs-SA)能与生物素化二抗IgG结合的特点进行免疫荧光组织化学检测细胞角蛋白(CK)和增殖细胞核抗原(PCNA)的蛋白表达,评价蛋白的定位是否准确,并每隔1周在荧光显微镜下观察荧光信号的瘁灭情况。结果:观察到CK、PCNA蛋白分别定位于肺癌细胞的胞膜和胞核,定位准确,并且阳性信号呈现橙红色的光。直到4周后,才观察到荧光信号有明显的减弱。结论:QDs具有很好的耐光性,很宽的激发和发射光谱,荧光寿命也长。采用链霉亲合素修饰的QDs,能精确地检测肺癌组织芯片上不同蛋白的定位。

Application of Quantum Dots Immune Labelling Technique in Lung Cancer Tissue Microarray

Objective: To investigate different antigens detected by a novel labelled reagent-quantum dots(QDs) in the lung cancer tissue microarray(TMA).Methods: Because QDs streptavidin conjugate(QDs-SA) could be combined specially with biotinylation IgG,immunofluorescence histochemistry was utilized to examine expression of cytokeratin(CK) and proliferating cell nuclear antigen(PCNA) proteins in the lung cancer TMA,to determine whether or not the protein accurate location was evaluated.And the fluorescence signal photodegradation was observed by fluorescence microscope every other week.Results: CK and PCNA proteins were located accurately in the cell membrane and nucleus of lung cancer cells,respectively,and positive signals showed salmon pink light.Until four weeks later,fluorescence signal was manifested significantly attenuated.Conclusion: QDs exhibit excellent photostability,broad emission spectrum and long fluorescence lifetime.QDs modified with streptavidin could accurately detect different protein locations in the lung cancer TMA.