The anti-tumor effect of a polypeptide extracted from Tegillarca granosa Linnaeus on renal metastatic tumor OS-RC-2 cells |
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Authors: | Wenhua Xu Xiaoying Kong Changqing Jiang Xiaoyan Liu Luo Xu |
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Affiliation: | 1.The Medical College of Qingdao University, Qingdao, China;2.Qingdao Agricultural University, Qingdao, China;3.Department of Pathology, Municipal Hospital of Qingdao, Qingdao, China;4.The Garrison Command Hospital of Qingdao, Qindao, China |
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Abstract: | IntroductionRenal cell carcinoma is a type of malignant tumor often diagnosed in the urinary system. The aim of this study is to evaluate the anti-tumor effects and mechanisms of a polypeptide, Haishengsu (TG-1), with different concentrations (125, 250, 500 mg/kg) on renal metastatic tumor OS-RC-2 cells.Material and methodsWe first established the renal metastatic tumor model. After being administered with TG-1, the weight of tumors was measured and the microstructural changes of renal carcinoma OS-RC-2 cells were compared using transmission electron microscopy before and after the therapy. The Ki67 expression in renal carcinoma OS-RC-2 cells was analyzed by RT-PCR and downstream signal molecule caspase-3 was measured by Western blot assay.ResultsAfter treatment with different doses of TG-1, the tumor weights in the positive control group and experimental groups were smaller than those in the untreated control group, suggesting that TG-1 could effectively inhibit tumor growth in mice. The transmission electron microscopy and flow cytometry results indicated that TG-1 induces tumor cell apoptosis (p < 0.05). The tumor cells exhibited polymorphism changes and chromatin edge clustering. TG-1 also inhibited Ki67 expression and promoted caspase-3 expression in the tumor significantly (p < 0.05).ConclusionsTG-1 inhibits the growth of the tumor and induces apoptosis of the tumor cells by inducing caspase-3 expression. The results provide a basis for clinical application of TG-1 in the future. |
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Keywords: | polypeptide renal tumor OS-RC-2 cell Ki67 mRNA anti-tumor effect |
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