植物源复杂基质中70种有机磷农药残留分析

目的 建立鲜香菇、大葱、韭菜、生姜等复杂基质中70种常用有机磷农药的SPE-气质联用分析方法。方法 样品经乙腈提取后, 用Envi-Carb II/PSA(石墨化炭黑/乙二胺基-N-丙基)复合小柱净化,最后用GC-MS测定。结果 在0.01~1.0 mg/kg添加浓度范围内平均回收率为60%~119%, 相对标准偏差(RSD)为1.5%~17.8%。其中68种有机磷农药的定量限为0.01 mg/kg, 灭线磷和特丁硫磷为0.005 mg/kg。结论 该研究符合残留分析和各国残留限量要求, 对保障食品安全, 促进食品出口具有重要的意义。

Determination of 70 organophosphorous pesticides in complex matrix of natural resources of plants

Objective To establish a method for the detection of 70 organophosphorous pesticides in multi-matrix such as fresh mushroom, welsh onion, chinese chive, ginger by solid phase extraction(SPE)-gas chromatography-tandem mass spectrometry. Methods The samples were extracted with acetonitrile and then purified by an Envi-Carb/PSA-solid phase extraction(SPE)column. The analytes were determined by gas chromatography-mass spectrometry. Results The recoveries were 60%~119% at the spiked levels of 0.01 (0.005)~1.0 mg/kg and the relative standard deviations(RSDs)were in the range of 1.5%~17.8%. The limits of quantitations (LOQs) of ethoprophos and terbufos were 0.005 mg/kg and others were 0.01 mg/kg. Conclusion The method can meet the pesticide residue limit requirements for all countries. This method has an important significance for ensuring food safety and stimulating food export.