| 活体小鼠中单个红细胞的拉曼光谱分析 |
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| 引用本文： | 孟令晶,林漫漫,牛丽媛,刘军贤,王何健,姚辉璐.活体小鼠中单个红细胞的拉曼光谱分析[J].分析化学,2011(9):1394-1399. |
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| 作者姓名： | 孟令晶 林漫漫 牛丽媛 刘军贤 王何健 姚辉璐 |
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| 作者单位： | 广西科学院生物物理实验室;广西师范大学物理科学与技术学院;广西科学院生物工程中心; |
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| 基金项目： | 国家自然科学基金(No.60868002); 广西自然科学基金(No.2011GXNSFA08143); 桂科院基金(No.11YJ24WL02); 广西科技攻关项目(No.0718007-30)资助 |
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| 摘 要： | 应用拉曼光谱与光镊技术对活体小鼠毛细动脉和静脉血管中的全血以及毛细血管中的红细胞和体外的全血及单个红细胞进行研究；光谱分析显示,可以获得活体血液的拉曼光谱；在动脉毛细管和静脉毛细管中可以观察到清晰的携氧与去氧血液的不同光谱；体内血管中血红细胞的血红蛋白浓度比体外的更高,在动脉毛细管中的血红细胞的携氧态特征峰明显,且具有...
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| 关 键 词： | 拉曼光谱 单个红细胞 活体 |
Raman Spectroscopic Analysis of Single Red Blood Cells in Vivo Rat |
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| MENG Ling-Jing,LIN Man-Man,NIU Li-Yuan,LIU Jun-Xian,HUANG He-Jian,YAO Hui-Lu.Raman Spectroscopic Analysis of Single Red Blood Cells in Vivo Rat[J].Chinese Journal of Analytical Chemistry,2011(9):1394-1399. |
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| Authors: | MENG Ling-Jing LIN Man-Man NIU Li-Yuan LIU Jun-Xian HUANG He-Jian YAO Hui-Lu |
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| Affiliation: | MENG Ling-Jing1,2,LIN Man-Man1,NIU Li-Yuan1,LIU Jun-Xian2,HUANG He-Jian3,YAO Hui-Lu1 1(Laboratory of Biophysics,Guangxi Academy of Sciences,Nanning 530003) 2(College of Physics and Technology,Guangxi Normal University,Guilin 541004) 3(Biotechnology Center,Nanning 530003) |
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| Abstract: | Raman spectroscopy was used to study red blood cells(RBCs) and blood in vein capillary,in artery capillary in vivo and in vitro with the use of optical tweezers.Results showed that,(1) We could get Raman spectroscopy of blood in vivo;(2) We could clearly observe that different blood spectra in oxygenated and deoxygenated state in artery and vein capillary;(3) By studying Raman spectroscopy of single RBC,we concluded that the hemoglobin concentration in capillary was larger than that in vitro,and oxygenated ... |
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| Keywords: | Raman spectroscopy Single red blood cells In vivo |
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