骨关节炎软骨退变与Krüppel样因子-5和基质金属蛋白酶-9表达的关系

目的 观察Krüppel样因子-5(KLF-5)和基质金属蛋白酶-9(MMP-9)表达活性与人膝关节软骨血管及神经纤维侵入之间的关系.方法 从 20例行全膝关节置换术的患者中,选取切除的31个关节标本;将每个标本切割成2～3块,并分成磨损组(n=29)和无磨损组(n=17).结果 在磨损组的关节软骨潮线附近有血管化发生,扫描电镜也证实血管结构的存在;关节软骨的神经侵袭仅发生于血管化的标本;与无磨损的关节软骨区比较,磨损区的血管化和神经支配数量明显增加(P<0.05).在磨损组的关节软骨中,KLF-5、MMP-9的表达活性明显高于无磨损组(P<0.05),而且与血管侵袭程度正相关.结论 增加的KLF-5和MMP-9表达可能是导致骨关节炎软骨退变和血管化的重要机制之一.

Abstract：

Objective To investigate the relationship between the expression neurovascular invasion in osteophytes in osteoarthritis (OA). Methods Thirty-one articular cartilage samples were collected from 20 patients who had undergone total knee arthroplasty (TKA), and each sample was divided into two groups: no change (NC,n=17) and severe change (SC,n=29) according to Mankin score and safranin O staining. Neurovascular markers protein gene product (PGP) 9.5 and CD34, and the expression of KLF-5, MMP-9 were detected by using immunohistochemistry. Results Vascular channels were observed in both NC and SC sections. In SC sections, 16/29 (55.2%) sections displayed vessels entering the calcified cartilage, which was more than that in NC group (2/17, 11.8%,P<0.05). The frequency of neurovascular invasion was significantly different between SC and NC (P<0.05). Innervation always accompanied vascular invasion at the osteochondral junction. The severity of neurovascular invasion was positively correlated with the expression of KLF-5 and MMP-9 in chondrocytes at the same site that was significantly different between SC and NC. Conclusion KLF-5-induced MMP-9 expression may be involved in cartilage matrix degradation and vascularization.