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| DGGE法在盛夏习酒酒醅的微生物菌群结构解析中的应用 | |
| 引用本文： | 施思,邓宇,李波,李中泰,唐云容,毛晓红,张文学.DGGE法在盛夏习酒酒醅的微生物菌群结构解析中的应用[J].酿酒科技,2010(3):51-53. |
| 作者姓名： | 施思 邓宇 李波 李中泰 唐云容 毛晓红 张文学 |
| 作者单位： | 1. 四川大学轻纺与食品学院,四川,成都,610065 2. 农业部沼气科学研究所,四川,成都,610041 3. 贵州茅台酒厂(集团)习酒有限责任公司,贵州,习水,564622 |
| 摘 要： | 利用PCR—DGGE技术分析了酒醅入窖前后的菌群变化。结果表明，发酵前微生物群落结构呈明显多样分布，发酵结束后优势菌明显改变，经同源性比较，Laetobacillus plantarum成为绝对优势菌，Weissella属细菌在酒醅中稳定存在。根据细菌菌群的变化及分布推测，在盛夏习酒（浓香型）酒醅发酵过程中，Lactobacillus和Weisselln细菌为重要菌群。 |
| 关 键 词： | 微生物 菌群 酒醅 16S rRNA DGGE |
Application of DGGE in the Analysis of Microflora Structure of Xijiu Fermented Grains in Summer |
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| SHI Si,DENG Yu,LI Bo,LI Zhong-tai,TANG Yun-rong,MAO Xiao-hong,ZHANG Wen-xue.Application of DGGE in the Analysis of Microflora Structure of Xijiu Fermented Grains in Summer[J].Liquor-making Science & Technology,2010(3):51-53. | |
| Authors: | SHI Si DENG Yu LI Bo LI Zhong-tai TANG Yun-rong MAO Xiao-hong ZHANG Wen-xue |
| Affiliation: | 1.Light Textile & Food Science College of Sichuan University/a>;Chengdu/a>;Sichuan 610065/a>;2.Biogas Scientific Research Institute of Ministry of Agriculture/a>;Sichuan 610041/a>;3.Xijiu Co.Ltd.of Maotai Distillery/a>;Xishui/a>;Guizhou 564622/a>;China |
| Abstract: | The change in microflora of fermented grains bef ore and after pit entry was analyzed by PCR-DGGE.The results showed that microflora structure displayed diverse distribution before the fermentation,after the fermentation,dominant bacteria changed evidently,Lactobacillus plantarum became the absolute dominant bacteria through homologous comparison,and Weissella existed stably in fermented grains.It was predicted according to the change in microflora and their distribution that Lactobacillus and Weissella wer... |
| Keywords: | microbe flora fermented grains 16S rRNA DGGE |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |
