反相高效液相色谱法测定抗病毒口服液中绿原酸和连翘苷含量

目的建立同时测定抗病毒口服液中绿原酸和连翘苷的反相高效液相色谱（RP—HPLC）法。方法采用岛津Shim-pack VP—ODS色谱柱（150mm×4．6mm，5μm），流动相为乙腈（A相）-0．02％醋酸（B相），0～20minA相的比例从5％线性增加至25％，流速为1mL／mim,检测波长为280nm。结果绿原酸和连翘苷均能良好分离，绿原酸质量浓度线性范围是0．116～1．160μmL，连翘苷质量浓度线性范围是0．65～6．50μg／mL，平均加样回收率分别为99．18％和99．36％，RSD分别为0．29％和0．20％（n=6）。结论RP—HPLC法简便、准确、重现性好．能排除其他成分的干扰．可用于该制剂的盾号控制．

Determination of Chlorogenic Acid and Forsythin in Kangbingdu Oral Solution by RP-HPLC

Objective To establish a RP- HPLC method for the determination of chlorogenic acid and forsythin in Kangbingdu Oral Solution. Methods The Shim-pack VP- ODS column （150 mm ×4.6 mm, 5 μm） was used. The mobile phase was acetonitrile and 0. 02% glacial acetic acid, the gradient program was acetonitrile 5% to 25% in 0- 20 min. The flow rate was 1 mL/mim. The detective wavelength was at 280 nm. Results Chlorogenic acid and forsythin could be separated completely. The calibration curves were linear within the range of 0. 116- 1. 160 μg/mL for chlorogenic acid, 0. 65-6. 50 μg/mL for forsythin, respectively. The average recovery rates were 99.18% and 99.36%, respectively, RSD was 0.29% and 0.20% （n =6）. Conclusion RP-HPLC method is easy, simple and accurate with good reappearance. It is the idea method for the quality control of Kangbingdu Oral Solution.