中药降乳散对大鼠催乳素瘤细胞表达 Bcl-2 、 Bax 的影响

 目的 研究中药降乳散对大鼠催乳素瘤细胞表达 Bcl-2 、 Bax 的影响。 方法 制备雌激素诱发大鼠催乳素瘤模型：成年雌性 Wistar 大鼠，切除卵巢，结扎子宫后随机分 3 组（ <> n =5 ）：对照组，皮下植入空白硅胶管；雌激素组，皮下植入含有己烯雌酚的硅胶管；降乳散组，皮下植入含有己烯雌酚的硅胶管，同时用中药降乳散汤剂（ 15 mg·kg ^{－ 1} ·d ^{－ 1} , 指干燥中药的质量）灌胃。 8 周后处死动物，垂体称重，用放免法测定血清催乳素水平。 Western blot 检测凋亡相关蛋白 Bax ， Bcl-2 在大鼠垂体组织中的表达。 结果 降乳散组的垂体质量、血清催乳素水平均明显低于雌激素组，具有显著差别（ <> P <0.01 ）。 Bax 在降乳散组的表达量明显高于雌激素组（ <> P <0.01 ）。 Bcl-2 在降乳散组的表达量明显低于雌激素组（ <> P <0.01 ）。 结论 中药降乳散具有促进催乳素瘤细胞凋亡的作用。本实验从分子水平探讨了中药抗催乳素瘤的机制，为中药治疗催乳素瘤提供了理论基础。

Effect of Jiangru Power on the Expression of Bcl-2 and Bax in Rat Prolactinoma

OBJECTIVE To study the effect of Jiangru Power on the expression of Bcl-2 and Bax in rat prolactinoma. METHODS Adult Wistar rats were ovariectomized and divided into 3 groups at random. Each rat in control group (n=5) was subscutaneously implanted with a blank implant. Rats in estrogen group（n=5） were implanted with estrogen-containing implants. Rats in Jiangru Power group（n=5）were implanted with estrogen-containing implants, meanwhile, Jiangru Power were adminstrated（15 mg·kg-1·d-1）. After 8 weeks, all the rats were executed. The pituitary gland was weighed. Serum prolactin (PRL) levels were measured by RIA method. The expression of Bcl-2 and Bax in pituitary tissue was measured by Western blot method. RESULTS Pituitary weights，PRL levels in Jiangru Power group were lower than those in estrogen group (P<0.001，respectively). The expression levels of Bcl-2 in Jiangru Power group were obviously lower than those in estrogen group (P<0.001). The expression levels of Bax in Jiangru Power group were obviously greater than those in estrogen group(P<0.001). CONCLUSION Jiangru Power may play a role in stimulating atoptosis in rat prolactinoma.