| 藏羚羊组蛋白去乙酰化酶1基因编码区的克隆与序列分析 |
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| 引用本文： | 刘芳,格日力.藏羚羊组蛋白去乙酰化酶1基因编码区的克隆与序列分析[J].兽类学报,2011,31(4). |
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| 作者姓名： | 刘芳 格日力 |
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| 作者单位： | 青海大学医学院高原医学研究中心,西宁,810001 |
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| 基金项目： | 国家自然科学基金资助项目(30660052) |
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| 摘 要： | 为探讨藏羚羊的低氧适应机制与高原低氧环境的相关性,采用RT-PCR技术,首次从藏羚羊心肌组织总RNA中克隆出组蛋白去乙酰化酶1(Histone deacetylase1,HDAC1)基因的编码区序列。该序列全长为1449bp,编码482个氨基酸,预测其蛋白质分子量约为55kDa。序列分析表明,藏羚羊HDAC1基因的编码区序列与其它哺乳动物相似性超过90%,其中与牛的相似性最高为98.27%;它的氨基酸序列与其它哺乳动物的相似性达到98.76%～99.59%,显示出极高的保守性。利用邻接法(Neighbor-Joining,NJ法)构建的分子系统进化树聚类结果表明,藏羚羊与牛先聚为一类,该聚类结果与传统的物种进化关系基本一致。藏羚羊HDAC1基因编码区的成功获得,为进一步揭示藏羚羊低氧适应的表观遗传机制奠定基础。
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| 关 键 词： | 藏羚羊 组蛋白去乙酰化酶1 基因克隆 低氧适应 |
Cloning and sequencing of HDAC1 coding cDNA sequence from Tibetan antelope(Pantholops hodgsonii) |
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| LIU Fang,GE Rili.Cloning and sequencing of HDAC1 coding cDNA sequence from Tibetan antelope(Pantholops hodgsonii)[J].Acta Theriologica Sinica,2011,31(4). |
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| Authors: | LIU Fang GE Rili |
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| Affiliation: | LIU Fang,GE Rili*(Research Center of High Altitude Medicine,Medical College of Qinghai University,Xining 810001,China) |
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| Abstract: | In order to explore the correlation between chronic hypoxia adaptation mechanism and a hypoxic environment at high altitude,the coding region of the HDAC1 gene was cloned for the first time from the total RNA of Tibetan antelope's cardiac muscle using RT-PCR.The encoding sequence was 1 449 bp in full length encoding 482 amino acids with a predicted molecular weight of about 55 kDa.Sequence analysis showed that the coding region of the HDAC1 gene cloned from the total RNA of Tibetan antelope shared more than... |
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| Keywords: | Gene cloning Histone deacetylase 1 Hypoxic adapation Tibetan antelope |
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