人参皂甙对顺铂所致大鼠肾损害的保护作用

通过药物与肾皮质薄片直接孵育法和注射给药法观察了人参皂甙（Ｇｉｎ）对顺铂（ＣＰ）所致大鼠肾损害的作用。结果表明：ＣＰ１．０ｍｍｏｌ·Ｌ￣（－１）在含有对氨基马尿酸（ＰＡＨ）的生理盐水溶液中与肾薄片孵育１２０ｍｉｎ，导致薄片中ＰＡＨ的蓄积明显减少，Ｇｉｎ８０ｍｇ·Ｌ￣（－１）增加ＰＡＨ的蓄积并抑制ＣＰ引起的ＰＡＨ蓄积减少。ＣＰ７．５ｍｇ·ｋｇ￣（－１）ｉｐ导致时间依赖性的血浆尿素氮（ＢＵＮ）升高，肾皮质组织中超氧化物歧化酶（ＳＯＤ）活性降低和脂质过氧化代谢产物丙二醛（ＭＤＡ）升高Ｇｉｎ５０ｍｇ·ｋｇ￣（－１）每日２次ｉｐ抑制ＣＰ所致的ＢＵＮ和ＭＤＡ升高及ＳＯＤ活性降低，推测Ｇｉｎ对ＣＰ所致大鼠肾损害的保护作用可能与其增强肾小管细胞的内在反应性和抗脂质过氧化作用有关。

Protective effects of ginsenosides against renal injuriesinduced by cisplatin in rats

The protective effects of ginsenosides (Gin) oncisplatin (CP)-induced nephrotoxicity were investigated in ratrenal cortical slices in vitro. The effect of Gin on CP-inducedlipid peroxidation was also observed in vivo. CP (1 mmol·L￣(-1)) significantly decreased hoth paminohippurate (PAH) ac-cumulation and ATP level. Gin (80 mg ·L￣(-1)) increascd PAHaccumulation and partially depressed the effects of CP. Inrats, CP (7.5 mg ·kg￣(-1)) induced a timedependent rise ofblood urea nitrogen (BUN). increase of inalondialdehyde(MDA) content and decline of superoxide dismutase (SOD)activity. Gin (50 mg · kg￣(-1) twice a day ip) inhibitcd CP-in-duced increase of BUN and MDA level and rcduction of SODactivity. This result might indicate that CP-inducednephrotoxicity was correlated to the generation of free radi.cals and subsequent lipid peroxidation. In addition. theanti-lipid peroxidation of Gin may also play an importantrole in nephrotoxicity induced by CP.