超高效液相色谱-串联质谱法同时测定粮食及肉制品中的24种工业染料

建立了测定粮食及肉制品中24种禁用工业染料的超高效液相色谱-串联质谱法。样品经甲醇、乙腈提取后,过WAX固相萃取柱净化。采用ACQUITY UPLC BEH C18分析柱分离,以10 mmol/L乙酸铵-0.2%甲酸水溶液和甲醇-乙腈(7:3, v/v)为流动相梯度洗脱,多反应监测(MRM)模式测定。24种染料的相关系数R²均大于0.99,回收率在61%~116%之间,方法的精密度(RSD, n=6)小于13%,定量限在0.1~4.0 μg/kg。该方法灵敏度高,操作简单、高效,适合于粮食及肉制品中24种禁用工业染料的同时定量及确证分析。

Simultaneous determination of 24 industrial dyes in grain and meat products by ultra performance liquid chromatography-tandem mass spectrometry

An ultra performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) analytical method was established for the simultaneous determination of 24 forbidden industrial dyes in grain and meat products. The sample was extracted with methanol and acetonitrile, and cleaned-up by a WAX solid phase extraction column. The solution was separated on an ACQUITY UPLC BEH C18 column eluted with a mixture of 10 mmol/L ammonium acetate-0.2%formic acid aqueous solution and methanol-acetonitrile (7:3, v/v) as the mobile phases, and then analyzed in multiple reaction monitoring (MRM) mode. The correlation coefficients were above 0.99, the average recoveries were 61%-116%, and the relative standard deviations (RSD, n=6) were lower than 13%. The quantification limits were 0.1-4.0 μg/kg. This method is simple, effective, sensitive, and suitable for the determination and confirmation of the 24 forbidden industrial dyes in grain and meat products.