| 苦瓜皂甙快速分离纯化方法研究 |
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| 引用本文： | 陈勋,于海宁,唐德松,洪军,沈生荣.苦瓜皂甙快速分离纯化方法研究[J].食品科学,2004,25(2):114-117. |
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| 作者姓名： | 陈勋 于海宁 唐德松 洪军 沈生荣 |
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| 作者单位： | 浙江大学农业与生物技术学院,浙江,杭州,310029 |
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| 摘 要： | 用乙醇浸提葫芦科植物苦瓜(Momordica charantia L.)，通过改变浸提液浓度，浸提时间和洗脱液浓度等，确定一种提取苦瓜总皂甙的优化工艺。用人参皂甙Rgt为对照品，通过选择最大吸收波长，绘制标准曲线定量测定苦瓜皂甙总量。
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| 关 键 词： | 苦瓜皂甙 乙醇 浸提 工艺优化 分离纯化 |
| 文章编号： | 1002-6630(2004)02-0114-04 |
Assay Study on Rapid Separation and Purification of Saponin Extract from Momordica charantia L. |
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| CHEN Xun,YU Hai-ning,TANG De-song,HONG Jun,SHEN Sheng-rong.Assay Study on Rapid Separation and Purification of Saponin Extract from Momordica charantia L.[J].Food Science,2004,25(2):114-117. |
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| Authors: | CHEN Xun YU Hai-ning TANG De-song HONG Jun SHEN Sheng-rong |
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| Abstract: | The rapid routine assay of separation and purification on the extraction of saponin by alcohol from Momordicacharantia L. was studied in this paper. The solvent concentration, extraction period and the ratio of elution solution were consideredto ensure an optimized technological process to extract and isolate saponin from Momordica charantia L. Rg1(from Ginseng) wastaken as control.From the maxmium absorbance wavelength selected between the two UV spectra of saponin from Momordicacharantia L. and Rg1, a standard curve of Rg1 was made to determine the total content of saponin in the extracted products. |
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| Keywords: | Momordica charantia L saponin separation and purification |
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