痛泻要方加减引经药防风对肠易激综合征大鼠内脏敏感性及脑肠轴不同靶点脑肠肽的影响

目的:研究痛泻要方加减防风对腹泻型肠易激综合征(D-IBS)大鼠内脏敏感性及脑肠轴不同靶点脑肠肽的影响。方法:将新生SD大鼠随机分为正常组、模型组、痛泻要方缺防风组、痛泻要方全方组,和痛泻要方倍用防风组,每组10只,除正常组外,其余各组采用母子分离结合乙酸灌肠法,复制腹泻型肠易激综合征大鼠模型,造模后各组灌胃给予相应药物,连续2周,检测粪便含水量和腹壁撤退反射(AWR),采用酶联免疫吸附法(ELISA)检测5-羟色胺(5-HT)和P物质(SP)的含量,通过免疫组化法检测促肾上腺皮质激素释放激素(CRH)的表达。结果:与模型组比较,各实验组均能显著降低粪便含水量、最小容量阈值和血浆、下丘脑、结肠中的5-HT,SP含量,减少CRH的表达(P0.05,P0.01)。痛泻要方全方组和痛泻要方倍用防风组大鼠粪便含水量,5-HT和SP含量及CRH的表达量较痛泻要方缺防风组明显减低,AWR评分明显升高,差异具有统计学意义(P0.05,P0.01)。结论:防风具有增强痛泻要方抑制腹泻型肠易激综合征的内脏高敏感性和调节脑肠轴不同靶点中的脑肠肽的效应。

Effect of Tongxie Yaofang with or Without Saposhnikoviae Radix on Colonic Visceral Hypersensitivity and Brain-gut Peptide in Irritable Bowel Syndrome Rats

Objective:To investigate the effect of Tongxie Yaofang with or without Saposhnikoviae Radix on colonic visceral hypersensitivity and brain-gut peptide in the diarrhea-predominant irritable bowel syndrome (D-IBS) rats. Method:Newly born SD rats were randomly divided into control group, model group, Tongxie Yaofang without Saposhnikoviae Radix group, Tongxie Yaofang group, Tongxie Yaofang with double Saposhnikoviae Radix group. Except for the control group, the rats in the other groups were separated from their mothers and given acetic acid by gavage to establish the D-IBS model. These rats in the each group were administered with corresponding drugs for two weeks. The score of abdominal withdrawal reflex (AWR) and the fecal moisture content in rats were measured. The contents of 5-hydroxytryptamine (5-HT) and P substance (SP) were determined by enzyme linked immunosorbent assay (ELISA). The expressions of corticotropin releasing factor (CRH) were determined by immunohistochemical technique. Result:The fecal moisture content, minimum volume threshold, 5-HT and SP levels and expression of CRH in experimental groups were significantly lower than those in model group (P<0.05, P<0.01). Compared with the Tongxie Yaofang without Saposhnikoviae Radix group, the fecal moisture content, 5-HT and SP levels and expression of CRH in the Tongxie Yaofang group and the Tongxie Yaofang with double Saposhnikoviae Radix group decreased significantly, while the AWR score increased in different degrees (P<0.05, P<0.01). Conclusion:Saposhnikoviae Radix can enhance the therapeutic effect of Tongxie Yaofang decoction in inhibitng visceral hypersensitivity and regulating brain-gut peptide at different targets in D-IBS rats.