Attenuation of GVHD for allo-bone marrow transplantation recipient by fasL-fas pathway in an H-2 haplotype disparate mouse combination |
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Authors: | Email author" target="_blank">Liu?LingboEmail author Zou?Ping Hu?Zhongbo Zhong?Zhaodong Xiao?Juan Guo?Rong Xu?Zhiliang |
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Affiliation: | Institute of Hematology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China |
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Abstract: | Summary In order to explore a new special and effective way to prevent, graft versus, host disease (GVHD) after allogenic bone marrow
transplantation (alle-BMT), the stem cell antigen-1 (Sea-1) carly hematopoietic cells (EHC) from BALB/c mouse (H-2d) were introduced with exogenous mouse Fas ligand (mFasL) cDNA gene by the retrevirus-mediated gene transfer and expanded
for one week, and then they were co-cultured with the spleen mononuclear cells (SMNC) from BAC mouse (H-2d, h) as one way mixed lymphocyte reaction (OWMLR). The cytotoxicity of treated BAC mouse SMNC against Na2
51CrO4 labeling SMNC from BALB/c mouse was observed. The bone marrow mononuclear cells (BMMNC) from BAC mouse treated by the above
methods were transplanted into lethally-irradiated congenic BALB/c mice to observe the occurrence of GVHD. The results showed
that the SMNC from BAC mouse after OWMLR with exogenous mFasL cDNA gene-transduced hematopoietic cells (HC) from BALB/c mouse
in a ratio of 1 to 5 exhibited an obvious inhibition of the cytotoxicity against the BALB/c mouse spleen cells at different
effector/target ratios as compared to the control group (P<0.01). The grade I GVHD or no GVHD and the 80% survival rate at day 60 post-BMT were observed in the BALB/c mouse receiving
BAC mouse BMMNC treated with similar way, while the grade II–III GVHD and the 20% survival rate were noted in the control
group (P<0.01). It is suggested that the attenuation of GVHD in allo-BMT recipient could be successfully achieved through FasL-Fas
pathway in an H-2 haplotype disparate mouse combination.
LIU Lingbo, male, born in 1964. Associate Professor
This project was supported by a grant from National Natural Sciences Foundation of China (No. 39770767). |
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Keywords: | Fas ligand Fas allogenic bone marrow transplantation graft versus host disease |
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