顺铂对HeLa细胞6-磷酸葡萄糖脱氢酶表达和活性的影响

目的：探讨化疗药物顺铂在体外对HeLa细胞6-磷酸葡萄糖脱氢酶(G6PD)mRNA表达和活性的影响。 方法:5 mg/L顺铂作用于HeLa细胞一段时间后，MTT法检测细胞的生长抑制情况。Beutler改良法测定胞内还原型谷胱甘肽的含量，速率法测定G6PD活性，采用实时荧光定量RT-PCR观察G6PD mRNA的表达水平。结果:顺铂在体外能明显抑制HeLa细胞的生长。顺铂作用4 h和24 h后，G6PD mRNA的表达水平增强，且G6PD活性高于未加药对照组（P＜0.05）。4 h实验组GSH含量低于对照组。结论:顺铂能诱导HeLa细胞G6PD mRNA表达增加，G6PD活性增强。

Effects of cisplatin on glucose-6-phosphate dehydrogenase expression and activity in HeLa cells

AIM: To investigate the effect of cisplatin (DDP) on the expression of glucose-6-phosphate dehydrogenase (G6PD) mRNA and G6PD activity in HeLa cells in vitro. METHODS: After treated with the concentration of 5 mg/L DDP for several hours, HeLa cells were harvested. The growth inhibition was detected by methyl thiazolyl tetrazolium (MTT) assay. The content of glutathione (GSH) was determined by Beutler method. The activity of G6PD was determined by velocity method. The expression of G6PD mRNA was assayed by fluorescent quantitative real-time PCR. RESULTS: DDP significantly inhibited the proliferation of HeLa cells in vitro. At both 4 h and 24 h, the expressions of G6PD mRNA were up-regulated and G6PD activity were higher than that in control group (P<0.05). The content of GSH in 4 h group was lower than that in control group. CONCLUSION: DDP up-regulates the expression of G6PD mRNA and increases G6PD activity. These maybe contributes to the resistance of oxygen stress.