黑豆凝集素的提取及血凝效果研究

采用70%饱和度硫酸铵盐析的方法,分别以蒸馏水、生理盐水、pH=7.2的磷酸缓冲液、pH=5.2的乙酸缓冲液和0.7mol/L的甲酸作为浸提溶液,提取黑豆凝集素粗品,透析并用分子筛层析纯化黑豆凝集素,以鸡血凝集试验检测.结果表明:磷酸缓冲液为最佳浸提液,黑豆凝集素提取率最高,为9.17%,凝血效果最好.血凝试验表明分子筛层析图谱中出现的第1个峰即为黑豆凝集素,冷冻干燥后所得产品为白色粉末.

On Extraction of Lectin from Black Soya Been and Its Effect of Cruor

Crude lectin was extracted from the seeds of black soya been with distilled water,normal saline,phosphoric acid buffer(pH7.2),acetic acid buffer(pH5.2) and 0.7 mol/L formic acid solution,and was purified with precipitation with 70%(NH4)2SO4,dialysis and molecular sieve chromatography.The change in agglutinating activity was tested with erythrocytes of chicken.The results showed that the treatment with phosphoric acid buffer solution agglutinated erythrocytes better and the extraction efficiency(9.17%) was higher than other groups.It was confirmed by agglutinating examination that the first peak in the chromatograph was the lectin.The lectin of black soya been was white powder after lyophilization.