| SD乳鼠海马神经细胞原代培养方法的探讨 |
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| 引用本文： | 张挺,于鸿儒,杨菁,李冬利,梁巍巍. SD乳鼠海马神经细胞原代培养方法的探讨[J]. 辽宁医学院学报, 2005, 26(5): 47-49 |
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| 作者姓名： | 张挺 于鸿儒 杨菁 李冬利 梁巍巍 |
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| 作者单位： | 锦州医学院生物化学教研室,辽宁,锦州,121001;锦州医学院生物化学教研室,辽宁,锦州,121001;锦州医学院生物化学教研室,辽宁,锦州,121001;锦州医学院生物化学教研室,辽宁,锦州,121001;锦州医学院生物化学教研室,辽宁,锦州,121001 |
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| 摘 要： | 目的 寻求一种简单、廉价的原代SD乳鼠海马神经细胞培养方法.方法 采用胰蛋白酶消化法制备游离海马神经细胞悬液,进行培养.结果 通过多次探索、观察、成功的进行了原代海马神经细胞培养.结论 本方法适合一般实验室开展海马神经细胞培养.
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| 关 键 词： | 海马神经元 原代培养 |
| 文章编号： | 1000-5161(2005)05-0047-03 |
| 收稿时间： | 2005-05-17 |
| 修稿时间： | 2005-05-17 |
Study on the Method of Primary Culture for Hippocampal Neurons of SD Newborn Rats |
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| ZHANG Ting,YU Hong-ru,YANG Jing,LI Dong-li,LIANG Wei-wei. Study on the Method of Primary Culture for Hippocampal Neurons of SD Newborn Rats[J]. Journal of Liaoning Medical University (LNMU) Bimonthly, 2005, 26(5): 47-49 |
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| Authors: | ZHANG Ting YU Hong-ru YANG Jing LI Dong-li LIANG Wei-wei |
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| Affiliation: | Department of Biochemistry, Jinzhou Medical College, Jinzhou 121001 China |
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| Abstract: | Objective To search for an easier and cheaper method for the primary culture for hippocampal neurons of SD newborn rats. Methods The primary culture of SD newborn rats monolayer hippocampal neurons was performed in a simple way, in which hippocampal neurons were digested by trypsin and cultured. Results The primary culture of hippocampal neurons in this way was performed successfully through many times of observatings. Conclusions The method for the primary culture of hippocampal neurons is a good method for the general labs. |
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| Keywords: | hippocampal neurons primary culture |
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