Muscarinic modulation of acetylcholine release from slices of guinea pig nucleus basalis magnocellularis |
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Authors: | Anna Siniscalchi Irene Badini Antonio Cintra Kjell Fuxe Clementina Bianchi Lorenzo Beani |
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Affiliation: | a Institute of Pharmacology, University of Ferrara, Ferrara, Italy b Karolinska Institutet, Department of Histology, Stockholm, Sweden |
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Abstract: | Spontaneous and electrically evoked endogenous acetylcholine release and [3H]-choline efflux from slices of guinea pig nucleus basalis magnocellularis (nbM) were studied. Tetrodotoxin reduced the spontaneous endogenous release by 55%, while the Ca2+-free medium reduced it by about 30%. Evoked [3H]-choline efflux was Na+ and Ca2+ dependent and frequency related. Physostigmine, 30 μM, nearly halved the stimulation-evoked efflux; atropine, 0.15 μM, not only antagonized, but even reversed this effect into facilitation. Pirenzepine, 1 μM, and AFDX 116, 1 μM, were less effective than atropine, and reversed the inhibitory effect of physostigmine only when applied together. 4-DAMP, 0.01 μM, was ineffective. These findings indicate that acetylcholine release in guinea pig nbM slices is inhibited by the cooperation of muscarinic autoreceptors, possibly belonging to the M1 and M2 subclasses. |
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Keywords: | Acetylcholine release Nucleus basalis magnocellularis Muscarinic autoreceptor |
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