煤工尘肺结核耐药菌的基因分析

目的　了解煤工尘肺结核患者所染结核杆菌对异烟肼、利福平、乙胺丁醇及链霉素耐药情况和基因突变情况,试图寻找有效的预防和诊疗方法。方法 从96份煤工尘肺结核患者痰标本中分离出64株耐异烟肼、利福平、链霉素及乙胺丁醇的结核杆菌,并用聚合酶链反应——单链构象多态性分析法(PCR-SSCP)扩增出katG、rpoB及rpsL片段,再将这些片段的8%非变性聚丙烯酰胺凝胶电泳图与标准株的电泳图进行对照分析。结果 常规药敏试验检测出各种耐药株共64株,经PCR-SSCP法分析,42株rpsL异常,47株rpoB异常,42株katG异常。结论 大部分煤工尘肺结核患者的结核杆菌耐药分离株有基因突变,其突变率低于常规药敏试验结果。

Analysis on gene of strains of drug-resistance of Mycobacterium tuberculosis isolated from coal worker's pneumoconiosis with tuberculosis

Objective To realize the gene mutation of Myco ba cterium tuberculosis strains resisted with isoniazid,rifampicin,ethambutol an d streptomycin isolated from coal workers' pneumoconiosis with tuberculosis in order to find more effective therapy and prevention methods.Methods 64 strains of isoniazid, rifampicin,streptomycin or eth ambutol resistant Mycobacterium tuberculosis were separa ted from 96 coal workers' pneumoconiosis with tuberculosis. Then we amplified t heir katG, rpoB or rpsL fragments of genome with PCR and compared their SSCP pro files with standard strains.Results Out of 64 drug-resistant strains,42 rpsL,47 rpoB and 42 katG SSCP profiles appe ared abnormal separately.Conclusion Most of the resistant strai ns in our analyse showed evidences on gene mutation.The mutation rate was lower than that of rutinc test.