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转反义ACS基因番茄和普通番茄果实中锌含量和钠钾比与抗病性的关系研究
引用本文:范蓓,申琳,刘灿,田世平,生吉萍.转反义ACS基因番茄和普通番茄果实中锌含量和钠钾比与抗病性的关系研究[J].光谱学与光谱分析,2009,29(2):492-495.
作者姓名:范蓓  申琳  刘灿  田世平  生吉萍
作者单位:1. 中国农业大学食品科学与营养工程学院,北京 100083
2. 中国科学院植物研究所,北京 100093
摘    要:植物激素与矿质元素吸收以及与植物抗性的关系研究是近年来人们关注的热点。实验采用电感耦合等离子体原子发射光谱法(ICP-AES)测定转反义ACS基因耐贮番茄(乙烯的生物合成被抑制)及同品种普通番茄果实(对照)中Zn,Na,K元素的含量及Na/K比例的差异,并对其与果实抗病原菌侵染能力的关系进行讨论。结果表明:乙烯生物合成受阻的转反义ACS番茄果实抗病原菌侵染的能力高于对照果实,其Zn元素的含量是普通番茄果实的1.5倍;K元素含量在两种果实中无明显差异,但Na元素含量显著高于对照,Na/K比值是对照的2.0倍。说明Zn元素含量和Na和K元素的比例关系可能在转反义ACS番茄果实抗病原菌侵染的过程中起到一定作用。

关 键 词:电感耦合等离子体-原子发射光谱    钠/钾  抗病性  转基因番茄  
收稿时间:2007-10-06

Relationship of Zn Content and Na/K Ratio to the Resistance to Pathogen Invasion in Antisense ACS Transgenic Tomato Fruit and Normal Tomato
FAN Bei,SHEN Lin,LIU Can,TIAN Shi-ping,SHENG Ji-ping.Relationship of Zn Content and Na/K Ratio to the Resistance to Pathogen Invasion in Antisense ACS Transgenic Tomato Fruit and Normal Tomato[J].Spectroscopy and Spectral Analysis,2009,29(2):492-495.
Authors:FAN Bei  SHEN Lin  LIU Can  TIAN Shi-ping  SHENG Ji-ping
Affiliation:1. College of Food Science and Nutritional Engineering, China Agricultural University, Beijing 100083, China2. Key Laboratory of Photosynthesis and Environmental Molecular Physiology, Institute of Botany, The Chinese Academy of Sciences, Beijing 100093, China
Abstract:In the present work,inductively coupled plasma atomic emission spectrometry(ICP-AES) was used to determine the content of Zn, Na and K and the ratio of Na/K in antisense ACS transgenic tomato fruit(in which the antisense ACC synthase gene construct was inserted) and in normal tomato fruit of the same variety(Lycopersicon esculentum cv.Lichun).The difference in mineral elements content between the two tomatoes and the involvement of Zn content and Na/K ratio in the disease resistance of postharvest tomato fr...
Keywords:ICP-AES  Zn  Na/K ratio  Disease resistance  Transgenic tomato  
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