大鼠脑损伤后经枕大池及立体定向脑内移植神经干细胞的对比研究

目的 探讨大鼠脑损伤后，将神经干细胞（neural stem cells，NSCs）经枕大池移植到蛛网膜下腔及立体定向移植到脑内，观察神经功能恢复情况。方法 体外培养的NSCs取自胎鼠皮层，采用Feeney自由落体脑创伤模型制成大鼠脑损伤模型，伤后24小时将NSCs经枕大池移植到蛛网膜下腔及经立体定向移植到脑内。伤前24小时、伤后24小时及1、2周行动物运动神经功能评分。结果 接受NSCs移植的两组大鼠神经运动功能评分均较损伤对照组明显提高（P〈0．05），两移植组大鼠神经运动功能评分无明显差别。结论 经枕大池移植的NSCs具有远距离迁移能力，并能像脑内移植一样明显有助于大鼠神经运动功能的恢复。

The contrast research on traumatic brain injury of rats by different transplanting ways of neural stem cells

Objective To investigate the different effects on the repair of adult rats experimental traumatic brain injury(TBI) when neural stem cells(NSCs) were injected into subarachnoid space by cisterna magna and into the brain directly by stereotaxis.Methods Neural stem cells(NSCs) derived from the cortex of rat embryoes were injected into the rats cisterna magna and into the brain directly by stereotaxis after 24h when the experimental traumatic brain injury rat models were made.The neurological motor function scores of rats were assessed at pre-injury 24h,post-injury 24h and after 1 and 2 weeks.Results There were significant improved neurological motor function scores in brain injured rats that received NSCs injection.There were no significant difference between the two transplanting ways.Conclusion Neural stem cells have the potential to migrate into injured brain via subarachnoid space and improve the injured neurological motor function of rats liking transplanting directly by stereotaxis.