共聚焦激光扫描显微镜活体观察针刺家兔双侧风池穴对大脑皮质内微循环的影响

目的 :探讨针刺穴位对微循环的影响。方法 :用共聚焦激光扫描显微镜在开放颅窗的动物模型上活体观察了针刺家兔双侧风池穴 ,对大脑皮质内微循环的影响。测量不同口径血管的轴流、缘流的荧光辉度 ,并描绘出荧光辉度曲线。测量同一血管在生理状态下针刺双侧风池穴引起的血液缘流厚度变化、血管口径变化 ,用SAS软件包做统计学分析 ,并用四分位法描图以间接观察血管运动。结果 :电针刺激能引起动脉的舒缩运动 ,但缘流厚度却无明显的变化或有所增加 ,又一次证明了血液缘流保护性屏障假说的真实性 ;电针刺激可引起血管口径为 31.36± 4.89μm的动脉明显的血管舒缩运动 ,对血管口径为 17.31± 2 .5 3μm和血管口径为71.0 0± 5 .30 μm的动脉则未引起血管口径的明显血管舒缩运动 ,在电针刺激各期中 ,以A5后遗效应期最明显 ,说明针刺除在针刺当时有效果 ,而在针刺结束后的后遗效应则可能发挥更大作用。结论 :针刺能通过引起大脑皮质内微循环状态的改变来实现其抗休克及治疗脑血管疾病的作用。

Observation on Circulation within Cerbral Cortex during Acupuncture on Rabbit's Fengchi Points under Confocal Laser Scanning Microscopy in Vivo

Objective: To study the effects of acupuncture on microcirculation. Method: The diameter of capillaries, the movement of diastole and systole of capillaries and the thickness of edge flowing blood in vessels were measured under confocal laser scanning microscopy(MRC600) in a rabbit opening cranial window perparation during acupuncture on rabbit's the Fengchi points.Results: Under the normality, acupuncture(or acupuncture combined with electronic stimulation) causes the movement of diastole and systole of capillaries within cerebral cortex of rabbit in vivid. The thickness of the edge flowing blood in the vessels remains along with the movement. And while acupuncture the most effective period is the later stage after pulling out the needles. Acupuncture can not cause the movement of diastole and systole on the microvessels that the diameter is 71.00 μm, This furnish evidence that there is the protective barrier formed by the edge flowing blood in vessels within cerebral cortex and is realted with the occurrence and development of cerebrovascular disease further.Conclusion: The results provided proof that acupuncture achieve good result while treat cerebrovascular disease was acupuncture can causes the movement of diastole and systole of capillaries within cerebral cortex of rabbit in vivid.