新疆不同民族喉乳头状瘤与喉癌组织中不同类型HPV的检测

目的：探讨新疆部分民汉族喉乳头状瘤（LP）和喉癌（LC）与人乳头状瘤病毒（HPV）感染的关系。方法：应用聚合酶链反应的（PCR）技术，对83例喉癌患者（维吾尔族27例，哈萨克族4例，汉族52例），63例喉乳头状瘤患者（维吾尔族33例，汉族30例），20例声带炎性息肉（LIP）患者（维、汉各10名），总共166例分别进行HPV6／11的9．6％（8／83）和LIP的0．0％（0／20）（P＜0．05）；（2）LC组织中HPV16／18感染的阳性率为37．3％（31／83）明显高于LP的6．3％（4／63）和LIP的0．0％（0／20（P＜0．05）；（3）民汉之间的差异无统计学意义（P＞0．05）。结论：（1）民汉LP的发生主要与HPV6／11感染有关；LC主要与HPV16／18感染关系密切，HPV感染可引起细胞基因的异常调控，可能是喉上皮组织发展成为肿瘤病变的促进因子。

Detection of HPV Infection in Laryngeal Papilloma and Laryngeal Carcinoma of the Patients of Various Ethnic Groups in Xinjiang

Objective: To evaluate the role of human papillomavirus (HPV) infection in laryngeal papilloma (LP) and laryngeal carcinoma (LC) of the patients of various ethnic groups in Xinjiang. Methods: HPV6/11?HPV16/18 were detected in 166 patients of biopsy and laryngectomy specimens with LP and LC of various ethnic groups in Xinjiang, including 63 cases of LP, 83 cases of LC and 20 cases of laryngeal inflammatory polyps (LIP) by polymerase chain reaction (PCR). Results: The positive frequency of HPV 6/11 infection was higher in LP(33.3%) than in LC (9.6%) and LIP(negative) (P<0.05); the HPV 16/18 infection was 37.3% in LC and showed higher than in LP (6.3%) and LIP (negative) (P<0.05),but no statistical significance was determined in the patients of various ethnic groups in Xinjiang (P>0.05). Conclusion: HPV infection may be an important tumorigenic factor and induce some cytogenetic abnormal regulations, HPV6/11 mainly involve benign laryngeal papillomatous lesions and HPV16/18 may implicate laryngeal carcinogenesis in laryngeal epithelial tissues to develop neoplastic lesions.