小鼠原位小肠移植模型的建立

目的:探索原住小肠移植的手术技术,为研究移植小肠功能及排斥反应提供良好的动物模型.方法:采用小肠供体的门静脉与受体下腔静脉端侧吻合,供体带主动脉片的肠系膜上动脉与受体腹主动脉端侧吻合,受体相应长度肠管切除,供体近、远端肠管与受体相应肠管切除后近、远端以端端吻合的方式建立原位小肠移植模型.术后禁食4d,不禁饮,每天分2次...

Mouse model of orthotopic small bowel transplantation

Objective To investigate the surgical procedures of orthotopic small bowel transplantation (SBT) model in mice to study the function and rejection of SBT.Methods We established a mouse SBT allograft model as follows: the donor portal vein was anastomosed end by side with the recipient inferior vena cava; the donor superior mesenteric artery with aorta patch was anastomosed end by side with recipient abdominal aorta. After an appropriate length of the recipient's small bowel was removed, the donor's small bowel and the recipient's small bowel were end-to-end anastomosed discontinuously. The mice were fasted for 4 d after the operation, free access to water and subcutaneously injection of 2 mL of 5% glucose saline twice daily. Operation success was regarded as survival for more than 5 d. There is no antibiotic and immunosuppressor.Results A total of 30 transplantations were done, the 5 d survival rate was 60% (18/30), and 12 died within 5 d. Among the dead recipients, 5 died of arterial anastomotic stenosis and anastomotic thrombosis, 2 of hemorrhagic shock caused by anastomotic bleeding, and the other 5 of intra-abdominal infection caused by postoperative intestinal fistula. The donor's operative time was (40±4.5) min, warm ischemia time was about 0.5 min, donor preparation time was about 3 min, and cold preservation time was (30±7.5) min. The recipient's operative time was (95±8.0) min, among which, the abdominal aorta and inferior vena cava clamping time was (38±3.5) min, the venous anastomotic time was (10±2.0) min and the arterial anastomotic time was (15±3.0) min. The mean intra-operative blood loss of the surviving recipient mice was about 0.2 mL.Conclusion High quality vascular anastomosis, and rehydration of donors and recipients are crucial factors for improving the success rate of SBT.