幼苗期淹水胁迫及喷施烯效唑对小豆生理和产量的影响

为探究幼苗期淹水胁迫及喷施烯效唑(S3307)对小豆(Vigna angularis)生理代谢和产量的影响,本研究于2018—2019年以龙小豆4号和天津红为试验材料,盆栽条件下,苗期进行预喷施S3307,同时连续淹水处理5 d并每天取样,测定分析相关生理指标。结果表明,幼苗期淹水胁迫引起小豆叶片H2O2和MDA含量、脯氨酸和可溶性蛋白含量显著提高,ABA、IAA和SA含量显著提高,SOD、POD和CAT活性显著提高,淹水处理5 d导致龙小豆4号单盆产量显著下降8.40%~12.61%,天津红显著下降9.91%~10.01%。S3307具有抵御淹水胁迫的作用,能有效增加小豆叶片脯氨酸和可溶性蛋白含量,显著降低H2O2和MDA含量,能够显著提高SOD和POD活性以及SOD/POD、SOD/CAT,显著增加ABA和SA含量,并显著抑制IAA含量的上升。喷施S3307使淹水4 d的龙小豆4号产量显著提高2.85%~6.18%,使天津红淹水4 d的产量显著提高2.85%~3.95%。综上,淹水胁迫下,不同品种小豆在活性氧物质积累、膜质过氧化、抗氧化酶活性以及激素水平等方面的生理应激存在显著差异;喷施S3307能够有效缓解淹水胁迫对小豆生理和产量的影响。本研究结果为进一步研究小豆苗期抵御淹水胁迫的生理机制及提高淹水胁迫下小豆的产量提供理论依据。

Effects of uniconazole and waterlogging stress in seedling stage on the physio logy and yield in adzuki bean

In order to explore the difference on the resistance physiology and yield and the mitigation effect of uniconazole(S3307)pretreatment under waterlogging stress,the pot experiment was conducted using Longxiaodou 4(LXD 4)and Tianjinhong(TJH).The anti-stress physiological indexes and yield were measured on 5th day after waterlogging stress and foliar spraying S3307 at seedling stage,and samples were taken every day.The results showed that leaf physiological indexes in waterlogging stress were changed during seedling stage,the content of H2O2,MDA,proline and soluble protein were significantly increased,as the same as the content of ABA,IAA,and SA,and the activities of SOD,POD,and CAT.Waterlogging treatment for five days resulted in a significant decrease in the output of LXD 4 in a single pot by 8.40%to 12.61%,and that of TJH decreased by 9.91%to 10.01%.S3307 had the effect of resisting waterlogging stress,and could effectively increase the content of proline and soluble protein in the leaf of adzuki bean,significantly reduced the content of H2O2 and MDA,significantly increased the activity of SOD and POD,the value of SOD/POD and SOD/CAT,significantly increased the ABA and SA content,and significantly inhibited the increase in IAA content.Foliar spraying S3307 significantly increased the output of LXD 4 by 2.85%to 6.18%under waterlogging treatment for four days,and significantly increased the yield of TJH by 2.85%to 3.95%,respectively.This study concluded that there were significant differences under physiological stress in reactive oxygen species accumulation,membrane lipid peroxidation,antioxidant enzyme activities and hormone levels among different varieties of adzuki bean under waterlogging stress.Spraying S3307 could effectively alleviate the effects of waterlogging stress on the physiology and yield in adzuki bean.The results provide a theoretical basis for further studying the physiological mechanism of adzuki bean resistance to waterlogging at the seedling stage and improving the yield of adzuki bean under waterlogging stress.