不同制剂诱导单个核细胞IL-23、IL-12亚基表达的对比分析

为探索并比较人类外周血单个核细胞(PBMC)IL-23、IL-12亚基表达的诱导剂和调控模式,分离正常人PBMC、单核细胞、CD4+、CD8+T淋巴细胞亚群,检测各种细菌、细菌产物、细胞因子、丝裂原对上述细胞IL-23和IL-12亚基表达的诱导作用,半定量RT-PCR方法测定各亚基的mRNA表达水平。结果显示,未经刺激的PBMC可表达IL-23p19和IL-12p35mRNA;脂多糖(LPS)和金黄色葡萄球菌(SAC)可上调PBMCIL-23和IL-12各亚基的表达;γ干扰素(IFN-γ)预处理后予LPS刺激可使单核细胞IL-23p19、IL-12p35和IL-12p40表达高于LPS直接作用的表达水平。植物血凝素(PHA)可促进CD4+、CD8+T细胞IL-23p19表达,而对IL-12p35、p40表达无调节作用。本文结果提示,IL-23的两个亚基的表达都处于紧密调控之下,易受各类诱导剂调节;人类外周血细胞IL-23p19的表达和调控模式与IL-12p35既有相似之处又存在不同点。

A comparative analysis on the expressions of IL-23 and IL-12 subunits on peripheral blood mononuclear cells induced by different inducers

To investigate the expressions of IL-23 and IL-12 subunits on peripheral blood mononuclear cells (PBMC) induced by different inducers and their regulation patterns, human PBMC, monocytes and the CD4+ and CD8+ T lymphocytes were isolated, and the effects of various bacteria, bacterial products cytokines and mitogens on the expressions of IL-23 and IL-12 subunits on these cells were evaluated by semi-quantitative RT-PCR. The results showed that IL-23 p19 and IL-12 p35 mRNA were expressed in fresh preparations of PBMC, and the expression of all the IL-23 and IL-12 subunits were up-regulated by lipopolysaccharide (LBS) and Staphylococcus aureus Cowan strain (SAC). The expression levels of IL-23 p19, IL-123 p35 and IL-12 p40 on the LPS-stimulated monocytes pretreated with IFN-γ were higher than those stimulated with LPS alone. PHA promoted the expressions of IL-23 p19 on CD4+ and CD8+ T cells, whereas the expressions of IL-12 p35 and p40 were minimally regulated by PHA. Our results suggest that both subunits of IL-23 are under tight regulation, and easily regulated by various regulators,the expression pattern and regulation mode of IL-23 p19 is similar to as well as distinct from that of IL-12 p35.