黄精多糖对骨质疏松性骨折大鼠骨代谢因子的影响

背景：骨质疏松性骨折主要原因是雌激素减少所形成的高转换型骨质疏松,采用雌激素虽能够有效地治疗,但长期雌激素替代治疗会引发诸多疾病。目的：观察黄精多糖对骨质疏松性骨折大鼠骨代谢因子的影响。方法：6月龄雌性SD大鼠,随机分为对照组（n=8）和骨质疏松性骨折组（n=36）。骨质疏松性骨折组行卵巢切除后建立骨质疏松性骨折动物模型,再以0.066mg/kg雌激素,400,200,100mg/kg黄精多糖进行灌胃,2d/次,连续35d。采用Elisa方法测量骨钙素、抗酒石酸酸性磷酸酶的表达。结果与结论：骨质疏松性骨折大鼠的骨钙素、抗酒石酸酸性磷酸酶的阳性表达活性升高,给予黄精多糖干预治疗后,高剂量的黄精多糖使骨钙素、抗酒石酸酸性磷酸酶的阳性表达活性下降,而在中、低剂量组干预效果不显著。说明高剂量黄精多糖对骨质疏松性骨折大鼠具有降低骨钙素、抗酒石酸酸性磷酸酶的阳性表达活性的作用,促进骨折愈合的作用。

Effect of polygonatum polysaccharide on bone metabolism cytokines in osteoporotic fracture rats

BACKGROUND：Onset of osteoporotic fracture is mainly resulted from reduction of estrogen that forms high-turnover osteoporosis,though this phenomenon can be treated effectively using estrogen,the long-term estrogen replacement therapy can cause many diseases.OBJECTIVE：To investigate the effect of polygonatum polysaccharide（PSP）on the cytokines of bone metabolism in the osteoporotic fracture rats.METHODS：Female,6-month aged SD rats were divided into the control（n=8）and osteoporotic fracture（n=36）groups.After the success model preparation,rats in the osteoporotic fracture group were randomly treated by 0.066 mg/kg estrogen,400,200 or 100 mg/kg PSP by intragastric administration,twice per day,for 35 successive days.The expressions of bone gla protein（BGP）and tartrate-resistant acid phosphatase（TRAP）were detected by ELISA.RESULTS AND CONCLUSION：BGP and TRAP levels were significantly increased in osteoporotic fracture rats.After PSP treatment,the BGP and TRAP levels were significantly decreased in the high dose PSP group.But intervention was not significant in medium and low dose PSP groups.It suggests that PSP can decrease BGP and TRAP expressions in the osteoporotic fracture rats.PSP can promote union of fracture.