肿瘤微环境中免疫与基质细胞相关基因在肺腺癌中的预后价值

目的:鉴别与肺腺癌预后相关的免疫与基质细胞基因。方法:501例肺腺癌基因表达数据来自癌症基因组图谱（TCGA）数据库。肺腺癌样本的免疫和基质评分从ESTIMATE数据库中获取。χ2检验分析基质、免疫评分与患者临床病理特征的相关性。采用t检验以及Cytoscape中的Mcode模块筛选差异表达基因（DEGs）。采用Kaplan-Meier法绘制生存曲线。结果:基质细胞评分与M分期显著相关（χ2=6.391，P=0.011）。免疫评分与M分期（χ2=4.769，P=0.029）、T分期（χ2=11.672，P=0.009）和总生存期（χ2=6.334，P=0.009）显著相关。基于免疫评分的DEGs有262个，基于基质评分的DEGs有391个。基因功能富集分析和蛋白-蛋白相互作用网络分析差异表达基因，富集分析表明，DEGs参与调节免疫应答、抗原结合、免疫应答和受体结合。生存分析表明POSTN（P=0.0181）、PLEK（P=0.0434）、LY86（P=0.0136）、HCK（P=0.0487）对肺腺癌患者具有预后价值。结论:基质细胞相关基因POSTN高表达与患者不良预后相关，免疫细胞相关基因PLEK、LY86、HCK高表达预示患者预后较好。

Prognostic value of immune and stromal cell-related genes in tumor microenvironment in lung adenocarcinoma

Objective: To identify prognostic stromal/immune-associated genes in lung adenocarcinoma（LUAD）. Methods: Lung adenocarcinoma gene expression data（501 cases） were obtained from The Cancer Genome Atlas（TCGA）database. Immune and stromal score of lung adenocarcinoma sample were extracted from ESTIMATE database. Chi-square test was used to analyze the correlation between stromal immune score and clinicopathological characteristics of patients. Differentially expressed genes（DEGs） were screened by t test and Mcode module in Cytoscape. Kaplan-Meier method was used to draw the survival curve. Results: Stromal scores were significantly associated with Metastasis stage（χ2=6.391，P=0.011）. Immune scores were significantly associated with Metastasis stage（χ2=4.769，P=0.029）， Tumor stage（χ2=11.672，P=0.009） and overall survival rate（χ2=6.334，P=0.009）. There were 262 differentially expressed genes（DEGs） based on immune scores and 391 DEGs based on stromal scores. Functional enrichment analysis and protein-protein interaction network assessments were employed to analyze differentially expressed genes. Functional enrichment analysis showed that DEGs were involved in regulation of immune response， antigen binding， immune response and receptor binding. Survival analysis showed that POSTN（P=0.0181）， PLEK（P=0.0434），LY86（P=0.0136） and HCK（P=0.0487） had prognostic value in patients with lung adenocarcinoma. Conclusion:High expression of the stromal cell-associated gene POSTN is associated with poor prognosis in patients. High expression of immune cell-related genes PLEK， LY86 and HCK predicted a good prognosis.