| 依波西隆蓝与蛋白质作用的共振光散射光谱及其分析应用 |
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| 引用本文： | 吴会灵,李文友,何锡文. 依波西隆蓝与蛋白质作用的共振光散射光谱及其分析应用[J]. 分析化学, 2003, 31(8): 989-991 |
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| 作者姓名： | 吴会灵 李文友 何锡文 |
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| 作者单位： | 1. 南开大学化学系,天津,300071;复旦大学化学系,上海,200433
2. 南开大学化学系,天津,300071 |
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| 基金项目： | 国家自然科学基金 (No .2 0 175 0 0 9)，教育部博士点基金 (No .2 0 0 2 0 0 5 5 0 0 2 )资助项目 |
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| 摘 要： | 微量蛋白质对依波西隆蓝的共振光散射产生增强作用，且增强程度与蛋白质浓度有良好的线性关系，由此建立了测定蛋白质的灵敏共振光散射分析方法。利用该方法测定合成样品和血清样品中的蛋白质，均获得了可靠的分析结果。
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| 关 键 词： | 依波西隆蓝 蛋白质 共振光散射光谱 血清 生物化学检验 |
Study of the Reactions of Proteins with Epsilon Blue by Resonance Light Scattering Technique and Its Analytical Application |
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| Wu Huiling ,,Li Wenyou ,He Xiwen. Study of the Reactions of Proteins with Epsilon Blue by Resonance Light Scattering Technique and Its Analytical Application[J]. Chinese Journal of Analytical Chemistry, 2003, 31(8): 989-991 |
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| Authors: | Wu Huiling Li Wenyou He Xiwen |
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| Affiliation: | Wu Huiling 1,2,Li Wenyou 1,He Xiwen *1 1 |
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| Abstract: | Based on the enhanced effects of proteins on the resonance light scattering of epsilon blue at 400 nm, a method has been established for the determination of proteins. The procedure is superior to some current methods for its simplicity, stability, high efficiency and high sensitivity, and has been used for synthetic samples and human serum samples satisfactorily. |
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| Keywords: | Epsilon blue protein resonance light scattering |
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