| 高效液相色谱法测定南、北五味子中木脂素含量 |
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| 引用本文： | 杨博,陈彤,李学龙,徐鹏,付绍平,鱼红闪,金凤燮. 高效液相色谱法测定南、北五味子中木脂素含量[J]. 大连轻工业学院学报, 2009, 28(2): 102-106 |
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| 作者姓名： | 杨博 陈彤 李学龙 徐鹏 付绍平 鱼红闪 金凤燮 |
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| 作者单位： | 大连工业大学辽宁省发酵工程重点实验室; |
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| 基金项目： | 国家自然科学基金资助项目(30470055);;辽宁省教育厅科学研究计划资助项目(20060161) |
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| 摘 要： | 建立了用高效液相色谱法测定南、北五味子中5种木脂素成分(五味子醇甲、五味子醇乙、五味子酯甲、五味子甲素和五味子乙素)的分析方法。以甲醇和水为流动相,采用梯度洗脱,对21个不同产地的南、北五味子样本进行了色谱分离,并利用标准物质、保留时间和紫外光谱对木脂素进行定性。结果表明,样品中各组分的色谱峰基本达到基线分离,该方法重复性好、灵敏度高,为评价南、北五味子中的木脂素活性成分提供了一种可靠的分析方法。
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| 关 键 词： | 南五味子 北五味子 高效液相色谱 木脂素 |
Determination of lignans in Fructus schisandrae sphenantherae and Fructus schisandrae shinensis by high performance liquid chromatography |
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| YANG Bo,CHEN Tong,LI Xue-long,XU Peng,FU Shao-ping,YU Hong-shan,JIN Feng-xieFermentation Engineering Key Laboratory of Liaoning Province,Dalian Polytechnic University,Dalian ,China. Determination of lignans in Fructus schisandrae sphenantherae and Fructus schisandrae shinensis by high performance liquid chromatography[J]. Journal of Dalian Institute of Light Industry, 2009, 28(2): 102-106 |
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| Authors: | YANG Bo CHEN Tong LI Xue-long XU Peng FU Shao-ping YU Hong-shan JIN Feng-xieFermentation Engineering Key Laboratory of Liaoning Province Dalian Polytechnic University Dalian China |
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| Affiliation: | YANG Bo,CHEN Tong,LI Xue-long,XU Peng,FU Shao-ping,YU Hong-shan,JIN Feng-xieFermentation Engineering Key Laboratory of Liaoning Province,Dalian Polytechnic University,Dalian 116034,China |
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| Abstract: | A method was developed for simultaneous determination of five lignans(schizandrin,schisandrol B,schisantherin A,deoxyschizandrin and schisandrin B) in Fructus schisandrae sphenantherae and Fructus schisandrae shinensis by high performance liquid chromatography.The twenty-one samples from different habitats were separated with methanol and water as mobile phase in a gradient elution mode,and the lignans were characterized by migration time and ultraviolet spectrum of the standards.The results showed that fiv... |
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| Keywords: | Fructus schisandrae sphenantherae Fructus schisandrae chinensis high performance liquid chromatography lignans |
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