Long-wavelength fluorescence of tyrosine and tryptophan solutions |
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Authors: | P Macías M C Pinto C Gutiérrez-Mérino |
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Affiliation: | Departamento de Bioquímica y Biología Molecular y Genética, Facultad de Ciencias Universidad de Extremadura, Badajoz, Spain. |
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Abstract: | We report in this paper the presence of fluorescence bands of tryptophan and tyrosine solutions centered above 550 nm. This long-wavelength fluorescence is of much lower intensity, (0.4-2.7)%, than the UV fluorescence of these aromatic aminoacids. The basic characteristic of these fluorescence bands are: (a) tyrosine: lambda em = 600 nm with two excitation peaks centered at 453 nm and 550 nm (b) tryptophan: lambda em = 675 nm with two excitation peaks centered at 455 and 560 nm. It has been found that irradiation of tyrosine solutions with a potent UV lamp promotes an important increase of absorption at 310 nm and above 400 nm. |
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