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| 喉鳞癌患者外周血CD45~(low)CD34~+KDR~+内皮祖细胞的检测及其临床意义 | |
| 引用本文： | 董龙宝,厉倩,徐根林,田振华,谭龙益. 喉鳞癌患者外周血CD45~(low)CD34~+KDR~+内皮祖细胞的检测及其临床意义[J]. 中国肿瘤生物治疗杂志, 2011, 18(3). DOI: 10.3872/j.issn.1007-385X.2011.03.015 |
| 作者姓名： | 董龙宝 厉倩 徐根林 田振华 谭龙益 |
| 作者单位： | 1. 中国人民解放军第455医院耳鼻咽喉头颈外科,上海,200052 2. 上海市第一人民医院宝山分院检验科,上海,200940 3. 复旦大学附属金山医院耳鼻咽喉头颈外科,上海,200540 |
| 基金项目： | 上海卫生局青年科技基金项目(No.2007Y43)~~ |
| 摘 要： | 目的:研究喉鳞癌患者外周血中循环CD45lowCD34+KDR+内皮祖细胞(endothelial progenitor cells,EPCs)的水平及其与喉鳞癌发生、发展的相关性。方法:收集2010年2月至2011年2月20例喉鳞癌患者外周血标本,10例健康志愿者外周血作为对照。流式细胞术检测CD45lowCD34+KDR+EPCs占外周血CD34+造血干细胞或外周血淋巴细胞的比例,实时定量PCR检测PBMC中KDR mRNA和CD133 mRNA的表达水平,ELISA与Griess法分别检测血清中VEGF和NO的水平。结果:临床Ⅱ期~Ⅳ期的喉鳞癌患者外周血CD45lowCD34+KDR+EPCs占CD34+造血干细胞的比例和占淋巴细胞的比例均较健康对照组明显升高[(55.4±11.4)%vs(21.0±5.8)%,P<0.05;(1.27±0.25)vs(0.25±0.09),P<0.05]。临床Ⅱ期~Ⅳ期喉鳞癌患者PBMC中KDR mRNA和CD133 mRNA的表达水平及血清中VEGF水平均明显高于健康对照组(P<0.05),NO水平没有明显差异;而临床Ⅰ期的喉鳞癌患者CD45lowCD34+KDR+... |
| 关 键 词： | 喉癌 鳞癌 内皮祖细胞 血管内皮生长因子 |
Detection of CD45~(low) CD34~+ KDR~+ endothelial progenitor cells in laryngeal squamous carcinoma patients and their clinical significance |
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| DONG Long-bao,LI Qian,XU Gen-lin,TIAN Zhen-hua,TAN Long-yi. Detection of CD45~(low) CD34~+ KDR~+ endothelial progenitor cells in laryngeal squamous carcinoma patients and their clinical significance[J]. Chinses Journal of Cancer Biotherapy, 2011, 18(3). DOI: 10.3872/j.issn.1007-385X.2011.03.015 | |
| Authors: | DONG Long-bao LI Qian XU Gen-lin TIAN Zhen-hua TAN Long-yi |
| Affiliation: | DONG Long-bao1,LI Qian2,XU Gen-lin3,TIAN Zhen-hua1,TAN Long-yi2(1.Department of Otolaryngology,No.455 Hospital of PLA,Shanghai 200052,China,2.Department of Clinical Laboratory,Baoshan Branch of Shanghai First People's Hospital,Shanghai 200940,3.Department of Otolaryngology,Jinshan Hospital Affiliated to Fudan University,Shanghai 200540,China) |
| Abstract: | Objective: To study the ratio of circulating CD45low CD34+ KDR+ endothelial progenitor cells(EPCs) in peripheral blood of laryngeal squamous carcinoma patients,and to investigate its relationship with the development and progress of laryngeal squamous carcinoma.Methods: Blood samples from 20 laryngeal squamous carcinoma patients,who were diagnosed from Feb.2010 to Feb.2011,and 10 healthy volunteers were included in the present study.The ratio of CD45low CD34+ KDR+ EPCs in peripheral CD34+ haemopoietic stem ... |
| Keywords: | laryngeal carcinoma squamous cell carcinoma endothelial progenitor cells VEGF |
| 本文献已被 CNKI 万方数据 等数据库收录！ | |
