抗线粒体抗体阳性患者的AMA-M2亚型检测及ANA核型分析

目的了解不同滴度抗线粒体抗体(AMA)阳性患者AMA M2亚型抗体(AMA-M2)浓度分布状况及在原发性胆汁性肝硬化(PBC)患者中的阳性率及相伴ANA核型。方法采用间接免疫荧光法检测自身抗体,采用酶联免疫吸附试验检测502例AMA阳性标本中的AMA-M2,并对已明确诊断的71例PBC患者进行AMA-M2亚型及ANA核型分析。结果在502例AMA阳性患者中AMA-M2阳性率为76.1%;71例PBC中95.8%AMA抗体效价大于1:160、90.1%AMA抗体效价大于1:320、73.2%AMA抗体效价大于1:640。95.8%AMA-M2阳性:67.6%M2200 RU/ML、84.5%M2100 RU/ML,并有56.3%存在ANA。结论 PBC中AMA、AMA-M2呈高滴度、高浓度集中,高效价AMA、AMA-M2及ANA的部分核型有助于PBC的诊断。

The analysis of AMA-M2 subtype and karyotype in consecutive patients with postive anti-mitochondria antibodies

Objective To study the concentration distribution of AMA-M2 and explore the concomitant karyotype in patients with different titers of anti-mitoehondria antibodies. Methods The autoantibodies in specimen were measured by indirect immuno-fluorescence assay(IIF), AMA-M2 subtype in 502 specimens with positive anti-mitochondria antibodies was measured by ELISA, and the AMA-M2 and ANA karyotype in 71 patients with primary biliary cirrhosis were analyzed. Results The positive rates of AMA-M2 subtype is 76.1% in 4258 consecutive specimen including 502 specimens with positive anti-mitochondria antibodies. Of the 71 patients with PBC,95.8% of them have positive anti-mitochondria antibodies with titers above 1:160,90. 1% above 1 : 320, 73.2% above 1 : 640.95.8% patients have positive AMA-M2 subtype including 67. 6% above 200 RU/ML,84.5% above 100 RU/ML. 56.3% patients have positive ANA. Conclusion The positive of AMA and AMA-M2 in patients with primary biliary cir-rhosis appears high concentration. High titer of AMA, AMA-M2 and specific karyotype of ANA contributes to the diagnosis of PBC.