Enhancement of monoclonal antibody productivity by promoting active hypothermic growth in hybridoma cells |
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| Authors: | Sun Li Chong Duen Gang Mou Saw Hoon Lim Abdul Manaf Ali Beng Ti Tey |
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| Affiliation: | 1. Biotechnology Program, Malaysia University of Science and Technology, Dataran Usahawan Kelana Jaya, 17, Jalan SS7/26, Kelana Jaya 47301, Selangor, Malaysia;2. Institute of Bioscience, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia;3. Department of Cell and Molecular Biology, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia;4. Department of Chemical and Environmental Engineering, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia |
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| Abstract: | BACKGROUND: Many reports have suggested that mild hypothermic culture conditions improve the specific monoclonal antibody (mAb) productivity of mammalian cells. The effect of active hypothermic growth on the mAb productivity of the hybridoma C2E7 was investigated. Hybridoma growth under hypothermic conditions (32 °C) was stimulated by supplementation of the culture medium with high serum concentrations (up to 30%). RESULTS: Specific and volumetric mAb productivity of a stimulated, active growth, mildly hypothermic hybridoma culture (30% FBS supplemented, 32 °C) were 1.38‐ and 1.34‐fold greater than the control culture (10% FBS supplemented, 37 °C). The enhanced specific mAb productivity under hypothermic conditions was associated with an increase in IgM mRNA levels during both the lag and early exponential phases of hypothermic growth. CONCLUSION: Stimulation of hybridoma growth under mildly hypothermic conditions increased both the specific and volumetric mAb productivity of hybridoma cells. Copyright © 2009 Society of Chemical Industry |
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| Keywords: | hypothermic growth hybridoma monoclonal antibody specific productivity |
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