Selectivity of potato tuber lipid acyl hydrolase toward long-chain unsaturated FA in esterification reactions with glycerol analogs in organic media |
| |
| Authors: | Praphan?Pinsirodom Email author" target="_blank">Kirk?L?ParkinEmail author |
| |
| Affiliation: | (1) Present address: Department of Agro-Industry, Faculty of Agricultural Technology, King Mongut’s Institute of Technology, Ladkrabang, 10520 Bangkok, Thailand;(2) Department of Food Science, University of Wisconsin, Babcock Hall, 1605 Linden Dr., 53706 Madison, WI |
| |
| Abstract: | FA selectivity of a Celite-immobilized potato lipid acyl hydrolase (LAH) in esterification reactions with long-chain FA, including
stearic acid (18∶0), oleic acid (18∶1), linoleic acid (18∶2), α-linolenic acid (18∶3), EPA (20∶5), and DHA (22∶6), and alcohol
co-substrates (n-propanol, isopropanol, 1,3-propanediol, and glycerol) was studied in isooctane. Immobilized LAH was selective for FA of greater
degrees of unsaturation (18∶3>18∶2>18∶1>18∶0) for all alcohol acceptors evaluated. Selectivity of LAH toward unsaturated C18 FA increased with an increase in water activity (a
w
) from 0.19 to 0.90 for n-propanol, isopropanol, and 1,3-propanediol as alcohol co-substrates. In contrast, with glycerol as the alcohol cosubstrate,
selectivity of LAH toward these unsaturated C18 FA increased with a decrease in a
w
from 0.90 to 0.19. In addition, immobilized LAH strongly discriminated against EPA and DHA for both 1,3-propanediol and glycerol
as alcohol co-substrates. |
| |
| Keywords: | Anhydrous esterification fatty acid selectivity lipid acyl hydrolase patatin unsaturated fatty acid |
| 本文献已被 SpringerLink 等数据库收录！ |
|
