饥饿胁迫对罗非鱼肝胰脏抗氧化能力的影响

为了研究饥饿胁迫对罗非鱼肝胰脏抗氧化能力的影响，选择规格、体质量基本一致的健康罗非鱼80尾，个体初始体质量约(11.52±2.85) g ，随机分为4个平行组，在饥饿胁迫第1、7 、14 天采集肝胰脏样品，测定丙二醛(MDA)含量、超氧化物歧化酶(SOD)和过氧化氢酶(CAT)活性以及总抗氧化能力(T-AOC)。结果表明：随饥饿时间的延长，罗非鱼肝胰脏MDA含量和T-AOC水平均先降低后升高，饥饿第14 天 MDA含量和T-AOC水平最高，并显著高于饥饿第1天 (P<0.05)。SOD和CAT活性随饥饿时间延长呈逐渐上升趋势，饥饿第14天显著高于饥饿第1天 (P<0.05)。表明随着饥饿胁迫时间延长，罗非鱼肝胰脏MDA含量增加，抗氧化酶活性和总抗氧化能力代偿性升高，肝胰脏氧化损伤加剧。

Effects of Starvation Stress on Antioxidant Capacity in Hepatopancreas of Tilapia (Oreochromis niloticus)

This study was conducted to evaluate the effects of starvation stress on antioxidant capacity in hepatopancreas of tilapia (Oreochromis niloticus). Eighty fishes with an average weight of (11.52±2.85) g were divided into four parallel groups, and were starved for 14 d, the hepatopancreas were collected for determining the content of malonaldehyde (MDA), the total antioxidant capacity (T-AOC) level, and the activities of superoxide dismutase (SOD) and catalase (CAT) on 1st, 7th and 14th day. The content of MDA and the level of T-AOC decreased at the beginning of the starvation, and then increased as the starvation time passed, with their peak on the 14th day of starvation, which were significantly higher than those in the 1st day of starvation (P<0.05). The activities of SOD and CAT increased gradually as the starvation time extended, the activities in the 14th day of starvation were significantly higher than those in the 1st day of starvation (P<0.05). The results indicated that the content of MDA, the activities of antioxidant enzyme and the level of T-AOC in hepatopancreas of tilapia could be increased, and the oxidative damage in hepatopancreas could be aggravated with the starvation time extended.