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| 脱细胞猪小肠黏膜下层支架复合软骨细胞构建组织工程软骨 | |
| 引用本文： | 吴迪,谢瑜,王少云,朱晓松,李青,董坚,李世和.脱细胞猪小肠黏膜下层支架复合软骨细胞构建组织工程软骨[J].中国组织工程研究与临床康复,2011,15(21). |
| 作者姓名： | 吴迪 谢瑜 王少云 朱晓松 李青 董坚 李世和 |
| 作者单位： | 1. 昆明医学院第一附属医院,骨科,云南省昆明市,650032 2. 昆明医学院第一附属医院,血液科,云南省昆明市,650032 3. 昆明医学院第一附属医院,生物治疗中心,云南省昆明市,650032 |
| 基金项目： | Social,Development,Plan,of,Yunnan,Province,No.2008ZC117M |
| 摘 要： | 背景:关节软骨损伤后无论是否施加干预,都难以达到满意的修复效果.目的:观察以猪自体软骨细胞为种子细胞复合脱细胞猪小肠黏膜下层构建组织工程软骨的可行性.方法:将培养至第3代的猪膝关节软骨细胞接种于小肠黏膜下层膜上,复合培养48 h,构建细胞-载体复合物,光学显微镜、扫描电镜观察软骨细胞在小肠黏膜下层膜上的生长情况.结果与结论:苏木精-伊红染色见细胞在小肠黏膜下层基质层表面呈单层或复层生长;免疫组织化学染色结果显示软骨细胞与小肠黏膜下层表面之间形成一条连续阳性表达条带;扫描电镜见软骨细胞在支架孔隙内贴壁良好生长. |
| 关 键 词： | 小肠黏膜下层 关节软骨 自体 组织工程软骨 猪 |
Tissue engineered cartilage constructed by acellular porcine small intestinal submucosa scaffold combined with autologous chondrocytes |
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| Wu Di,Xie Yu,Wang Shao-yun,Zhu Xiao-song,Li Qing,Dong Jian,Li Shi-he.Tissue engineered cartilage constructed by acellular porcine small intestinal submucosa scaffold combined with autologous chondrocytes[J].Journal of Clinical Rehabilitative Tissue Engineering Research,2011,15(21). | |
| Authors: | Wu Di Xie Yu Wang Shao-yun Zhu Xiao-song Li Qing Dong Jian Li Shi-he |
| Affiliation: | Wu Di1,Xie Yu2,Wang Shao-yun1,Zhu Xiao-song1,Li Qing1,Dong Jian3,Li Shi-he11Department of Orthopedics,2Department of Hematology,3Centre of Biotherapy,First Affiliated Hospital,Kunming Medical University,Kunming 650032,Yunnan Province,China |
| Abstract: | BACKGROUND:It is difficult to achieve a satisfactory repair effect on articular cartilage injury with or without intervention.OBJECTIVE:To study the feasibility of constructing tissue engineered cartilage using cultivated the autologous chondrocytes combined acellular porcine small intestinal submucosa(SIS)scaffold.METHODS:The third-generation chondrocytes from porcine knee joint were incubated on porcine SIS and cultured for 48 hours to construct a chondrocyte-scaffold complex.The growth of chondrocytes on... |
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| 本文献已被 CNKI 万方数据 等数据库收录！ | |
