| 阴道毛滴虫单克隆抗体的制备和鉴定 |
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| 引用本文： | 高兴政,毛伊丁.阴道毛滴虫单克隆抗体的制备和鉴定[J].中国寄生虫学与寄生虫病杂志,1992,10(2):139-142. |
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| 作者姓名： | 高兴政 毛伊丁 |
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| 作者单位： | 北京医科大学寄生虫学教研室，新疆维吾尔自治区地方病防治研究所，江西省九江医学专科学校，北京医科大学寄生虫学教研室，北京医科大学寄生虫学教研室，北京医科大学生物化学教研室 北京 100083，乌鲁木齐 830002，九江 332000，北京 100083，北京 100083，北京 100083 |
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| 摘 要： | 用阴道毛滴虫滋养体免疫BALB/c小鼠,取脾细胞与NS_1鼠骨髓瘤细胞融合,选择4株(2A2,2A4,2A12,2H9)杂交瘤细胞,证明能分泌效价高、特异性强的单克隆抗体(McAb)。亚类鉴定为IgG1(2A2,2A4),IgG2b(2A12),IgG3(2H9)。2H9株和其他3株McAb分别能抗阴道毛滴虫细胞核和细胞膜。除2H9株外均可凝集或杀伤虫体,但2A2、2A4和2A12株介导的细胞毒性分别是不依赖和依赖补体的。这4株McAb与杜氏利什曼原虫前鞭毛体、枯氏锥虫锥鞭毛体和蓝氏贾第鞭毛虫滋养体无交叉反应。
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| 关 键 词： | 阴道毛滴虫 单克隆抗体 抗原定位 细胞毒性 交叉反应 |
PREPARATION AND IDENTIFICATION OF ANTI-TRICHOMON AS VAGINALIS MONOCLONAL ANTIBODIES |
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| Gao Xing-Zheng,Mao Yi-Ding,Tang Zi-Hao,Yu Chao,Zhu Yong-Hong,Yang Fei-Yi.PREPARATION AND IDENTIFICATION OF ANTI-TRICHOMON AS VAGINALIS MONOCLONAL ANTIBODIES[J].Chinese Journal of Parasitology and Parasitic Diseases,1992,10(2):139-142. |
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| Authors: | Gao Xing-Zheng Mao Yi-Ding Tang Zi-Hao Yu Chao Zhu Yong-Hong Yang Fei-Yi |
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| Affiliation: | Department of Parasitology, Beijing Medical University. |
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| Abstract: | Hybridomas producing monoclonal antibodies (McAb) directed against Trichomonas vaginalis have been produced by fusing NSI myeloma cells with spleen cells of BALB/c mice immunized with Trichomonas vaginalis.IFA technique was used to test the binding activity of four McAbs produced.The McAb belonged to the IgG subtypes IgGl(2A2,2A4 McAb),IgG3 (2H9 McAb) and IgG 2b (2A12 McAb).Three McAbs,designated 2A2,2A4,2A12,reacted with a surface membrane component of live Trichomonas vaginalis.One (2A12) of them produced com-plement-dependent cytolysis of the parasites.Others (2A2.2A4) produced complement-independent cytotoxicity of the parasites.2H9 McAb which reacted with the nucleus of the organisms did not agglutinate the parasites.The four McAbs which did not have cross reaction with some protozoa of Zoomastigophorea species were specific antibodies against Trichomonas vaginalis.(Figs.1-3) |
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| Keywords: | Trichomonas vaginalis monoclonal antibodies cytotoxicity cross teaction IgG subtypes |
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