贝类生态综合养殖系统中细菌丰度的变化

2011年4—10月在福建云霄，对以贝类为主的生态综合养殖系统内的藻类池（A池）和贝类池（B池）逐月采样检测，并分析两池水体及B池沉积物中细菌丰度的时空变化规律.结果发现:水体中细菌丰度，B池为1.01×106～4.35×106 cell/mL；A池为1.09×106～6.61×106 cell/mL.A池和B池水体中细菌丰度没有明显的月变化规律，但昼夜变化明显.B池水体中细菌丰度与叶绿素a、总氮、总磷、温度和盐度的关系4—7月呈明显的正相关(r=0.984，n=4)；8月细菌丰度急剧下降，因采样期间有连续降雨，细菌丰度变化和降雨可能有一定的关系.A池水体中细菌丰度与叶绿素a呈显著负相关（r=-0.821，n=7），与温度呈显著负相关（r=-0.830，n=7），温度不是影响A池细菌增殖的主要因子.B池底泥样品中细菌丰度比水体中的低，主要可能是水交换和养殖贝类活动共同作用的结果.采样期间发现，在阴雨天气时池塘水面会有泡沫漂浮，细菌丰度高于其所在水体达2.25×107 cell/mL，其作用机制有待于进一步研究

The Temporal and Spatial Variation of Bacteria Abundance of Integrated Multi-trophic Aquaculture(IMTA) Systems for Shellfish

Based on monthly sampling and examining the water and sediments of multi-trophic aquaculture (IMTA) systems which cultured shellfish(B pond) and algae(A pond) from April to October 2011 in Yunxiao County,Zhangzhou,Fujian Province.The temporal and spatial variation of bacteria abundance of these TMTA systems was discussed.The results showed that the bacteria abundance in B pond water counted through DAPI staining-fluorescence microscopy ranged from 1.01?06 to 4.35?06 cell/mL,as for the A pond,the abundance ranged form 1.09?06 to 6.61?06 cell/mL.There was no significant monthly variation found for the bacteria abundances in the water of IMTA systems. However,thee rere was significant diurnal variation.The bacterial abundance in the B pond water from April to October was not significantly positive correlated to chlorophyll a,total nitrogen,total phosphorus, temperature and salinity.However,significant positive correlation was found between bacterial abundance and temperature from April to July (r=0.984,n=4).Bacterial abundance increased with the temperature from April to July,but it decresed sharply in August,probobly it was due to the continuous rainfall. The bacterial abundance in the A pond water from April to October was significantly negative correlated to chlorophyll a (r=-0.821,n=7) and temperature (r=-0.830,n=7).Temperature was not the major factor for the bacterial abundance in the A pond water. It was found that the bacterial abundance of the sediments was lower than that in the B pond water, it was probably due to the combined action of the water exchange and shellfish activity.At the sampling period,some bubbles was found on the water surface in the rainy days，the bacterial abundance in the bubbles,reached up to 2.25×107 cell/mL,much higher than the bacteria abundance in the water.However,the mechanism need to be further studied