道地产区甘草遗传多样性的ISSR分析

目的:对4个核心道地产区甘草的遗传多样性进行研究,分析其遗传变异程度,为探讨道地药材的遗传机制奠定基础。方法:采用ISSR分子标记技术研究来自4个产地155个甘草个体的遗传多样性。结果:8条引物共扩增出56条多态性条带,占总带数的75.68%,每条引物扩增多态位点百分率(PPB)在44.44%～100%。聚类分析显示,各产地野生品基本能够各聚一类,内蒙古、宁夏、甘肃甘草遗传特征较为稳定,陕西甘草的变异幅度较大。结论:不同道地产区甘草的遗传变异具有明显的地域性特征,甘草道地药材的形成具有遗传基础。

Analysis on Genetic Diversity of Licorice Glycyrrhiza uralensis from Geo-Authentic Product Areas by ISSR Markers

ISSR molecular markers were used to detect genetic diversity of licorice including 155 individuals from 4 Geo-Authentic Product Areas. Fifty-six polymorphic bands were obtained by 8 primers, accounting for 75.68% of the total, the percentage of polymorphic bands (PPB) of each primer was from 44.44% to 100%. Groups cluster analysis indicated that the wild licorice from each area could be clustered into one group, the individuals from Neimenggu, Ningxia and Gansu had apparent genetic characters, while the ones from Shaanxi had a genetic variation in regional pattern. The genetic variation of licorice from different Geo-Authentic Product Areas was regional, which laid the foundation for the formation of genuine medicinal herbs.