| 高效液相色谱-串联质谱法分离鉴定绿原酸及其相关杂质 |
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| 引用本文： | 田晨煦,徐小平,廖丽云,张洁,刘静,周莎.高效液相色谱-串联质谱法分离鉴定绿原酸及其相关杂质[J].色谱,2007,25(4):496-500. |
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| 作者姓名： | 田晨煦 徐小平 廖丽云 张洁 刘静 周莎 |
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| 作者单位： | 1.West China Pharmaceutical School of Sichuan University, Chengdu 610041, China;2.Chengdu Medical College, Chengdu 610083, China |
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| 摘 要： | 建立了高效液相色谱-串联质谱法（HPLC-MS/MS)分离和鉴定绿原酸及其相关杂质的方法。采用C18色谱柱(5 μm,4.6 mm×150 mm),乙腈-水（含0.1%甲酸）(体积比为8∶92)为流动相,经HPLC-MS/MS和HPLC-二极管阵列检测器在线检测,对工业绿原酸中的奎尼酸、咖啡酸、绿原酸同分异构体等8个相关杂质的结构进行了鉴定。
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| 关 键 词： | 高效液相色谱-串联质谱法 鉴别 绿原酸 相关杂质 |
| 文章编号： | 1000-8713（2007）04-0496-05 |
| 收稿时间： | 2007-01-15 |
| 修稿时间： | 2007-01-15 |
Separation and Identification of Chlorogenic Acid and Related Impurities by High Performance Liquid Chromatography-Tandem Mass Spectrometry |
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| TIAN Chenxu,XU Xiaoping,LIAO Liyun,ZHANG Jie,LIU Jing,ZHOU Sha.Separation and Identification of Chlorogenic Acid and Related Impurities by High Performance Liquid Chromatography-Tandem Mass Spectrometry[J].Chinese Journal of Chromatography,2007,25(4):496-500. |
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| Authors: | TIAN Chenxu XU Xiaoping LIAO Liyun ZHANG Jie LIU Jing ZHOU Sha |
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| Affiliation: | 1.West China Pharmaceutical School of Sichuan University, Chengdu 610041, China;2.Chengdu Medical College, Chengdu 610083, China |
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| Abstract: | A method using high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) was established for analysis and identification of chlorogenic acid along with its related impurities. A Gemini C18 HPLC column (4.6 mm x 150 mm,5 microm) was used with acetonitrile-water ( containing 0.1% formic acid) (8:92, v/v) as mobile phase. Eight related impurities of chlorogenic acid were identified and their structures were determined by using online HPLC-MS/MS and photodiode array detector (DAD). The method is quick, simple and can be used directly to identify the structure of unknown trace substances in the sample of chlorogenic acid. |
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| Keywords: | high performance liquid chromatography-mass spectrometry/mass spectrometry(HPLC-MS/MS) chlorogenic acid related impurities identification |
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