针灸对脑梗死大鼠患侧海马CA1区Bcl-2的影响及其与学习记忆的关系

目的观察针灸对脑梗死大鼠患侧海马CA1区Bcl-2的影响及其与学习记忆的关系,从细胞凋亡角度探讨针灸对脑梗死大鼠学习记忆能力、中枢神经系统的可塑性影响及其可能机制。方法采用大脑中动脉梗死模型,对照组及针灸组各24只,用Y-迷宫测试学习记忆能力;用免疫组化方法测试Bcl-2蛋白及凋亡指数。结果针灸组大鼠学会Y-迷宫所花的次数明显少于对照组（P〈0.05）;针灸组患侧海马CA1区Bcl-2蛋白阳性神经元数比对照组多（P〈0.05）;针灸组大鼠患侧海马CA1区的凋亡指数明显低于对照组（P〈0.05）。结论①针灸增加海马Bcl-2蛋白的表达,使Bcl-2在海马CA1区中占优势而使细胞受到保护,细胞凋亡减少,海马最终得以保存更多神经元而发挥其改善学习记忆功能的作用。②针灸能明显改善脑缺血大鼠的学习记忆能力。

The effect of acupuncture on Bcl-2 of hippocampal neurons in ischemia side and the relationship between these effects and learning and memory in cerebral infarction rats

Objective To observe the effect of acupuncture on Bcl-2 of hippocampal neurons in ischemia side and the relationship between these effects and learning and memory in cerebral infarction rats.Methods The model of cerebral infarction rats was induced by occlusion of the middle cerebral artery（MCAO） with clew.The ability of learning and memory were evaluated by Y-maze.The expression of Bcl-2 of hippocampal neurons was analyzed with immunohistochemistry.Results The score of Y-maze in control groups exceeded that of acupuncture groups（P0.05）.The positive expression of Bcl-2 in hippocampus CA1 region at acupuncture groups exceeded that of control groups.Apoptosis index in hippocampus CA1 region at control groups exceeded that of acupuncture groups.Conclusion The ability of learning and memory in cerebral infarction rats is improved by acupuncture,which may be accomplished by increasing Bcl-2 expression in hippocampus CA1 region,then protecting hippocampus cells from apoptosis.