液相色谱-串联质谱法测定腐乳中的生物胺

建立了一种使用液相色谱-串联质谱（LC-MS/MS）技术检测腐乳中9种生物胺的方法。样品经5%三氯乙酸水溶液提取后，进行液相色谱-串联质谱法测定，采用外标法定量。对检测方法进行方法学验证，9种生物胺在质量浓度为10～200 ng/mL范围内线性相关系数R2＞0.999，方法检出限为0.03 mg/kg，定量限为0.1 mg/kg，回收率在85%～101%之间，相对标准偏差（RSD）在0.9%～4.8%之间。该方法结果精密、准确、重复性好、操作简单，适用于腐乳中9种生物胺的测定。

Determination of biogenic amine in sufu by LC-MS/MS

A liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed for the determination of 9 biogenic amines in sufu. The sample was extracted by 5% trichloroacetic acid aqueous solution, determined by LC-MS/MS, and quantified by external standard method. The method was validated. The correlation coefficient R2 was more than 0.999 in the range of 10-200 ng/ml, the detection limit was 0.03 mg/kg, the quantitation limit was 0.1 mg/kg, recovery was 85%-101%, and relative standard deviation (RSD) was 0.9%-4.8%. The method had precision and accurate results, good repeatability and simple operation, and it was suitable for the determination of 9 biogenic amines in sufu.