Quantitative GC-ECD Analysis of Halogenated Metabolites: Determination of Surface and Within-Thallus Elatol of Laurencia obtusa |
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Authors: | Daniela Bueno Sudatti Silvana Vianna Rodrigues Renato Crespo Pereira |
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Affiliation: | (1) Departamento de Biologia Marinha, Universidade Federal Fluminense, Caixa Postal 100644, CEP 24001-970 Niterói, Rio de Janeiro, Brazil;(2) Departamento de Química Analítica, Universidade Federal Fluminense, Outeiro de São João Batista, s/n, Niterói, Rio de Janeiro, Brazil |
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Abstract: | Information on natural concentrations or variability of secondary metabolites in marine organisms may be important both to ecological/evolutionary and applied approaches. A gas chromatographic procedure with an electron capture detector (GC-ECD) was developed to quantify the sesquiterpenoid elatol at the surface and within-thalli of 70 specimens of the red seaweed Laurencia obtusa. The concentration of elatol was highest within-thalli 9.89 mg g−1 of L. obtusa, dry weight (d.w.)], compared to lower values found at the surface 0.006 mg g−1 of L.obtusa (d.w.), or 0.5–10.0 ng cm−2]. This method provides a rapid and inexpensive quantification of small quantities of elatol, and probably may also be used to quantify other halogenated compounds usually found in red seaweeds. |
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Keywords: | Red seaweed Secondary metabolites Sesquiterpene Elatol Quantification Halogenated secondary metabolites GC-ECD method |
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